Author/Authors :
Naroeni, Aroem university of indonesia - Insitute of Human Virology and Cancer Biology (IHVCB), Indonesia , Yuliawuri, Hartiyowidi university of indonesia - Insitute of Human Virology and Cancer Biology (IHVCB), Indonesia , Hartanto, Yuliar Budi university of indonesia - Insitute of Human Virology and Cancer Biology (IHVCB), Indonesia , Soedarmono, Yuyun Unit Transfusi Darah Pusat, Palang Merah Indonesia (PMI), Indonesia , Bela, Budiman university of indonesia - Insitute of Human Virology and Cancer Biology (IHVCB), Indonesia , Ibrahim, Fera university of indonesia - Insitute of Human Virology and Cancer Biology (IHVCB), Indonesia
Abstract :
Indeterminate results of serological HIV test have been found in Indonesia. The screening procedure is following the prescribed by WHO for screening of blood donors which is based on 3 different serological HIV test during screening of blood donors. Discordant results are interpreted as indeterminate. This research aims to identify GIV strains that previously difficult to determine, and to evaluate whether the HIV strains present in Indonesia could pass the existing screening system. RTPCR detection test of HIV RNA were conducted for 40 blood donors samples with indeterminate serological HIV-test after a confirmatory test using western blot. Preliminary results showed that 24/32 (75%) of the samples are positive LTR, 4/31 (12%) positive pol and 1/3 (33%) positive env. Amplification in p24 region showed that bands found have lower size than expected. Sequencing performed to confirm these findings show that further analysis is needed to determine whether this change is what behind the indeterminate results.
Keywords :
env , indeterminate , LTR , pol , p24
