Title of article :
A New,Rapid, Cost-Effective, Easy and ValidatedRP-HPLC Method for Determination of Antiviral(Sofosbuvir) in Bulk Forms
Author/Authors :
Mehmood, Yasir Department of Pharmaceutics - Faculty of Pharmaceutical Sciences - Government College University Faisalabad, Pakistan , Ullah Khan, Ikram Department of Pharmaceutics - Faculty of Pharmaceutical Sciences - Government College University Faisalabad, Pakistan , Shahzad, Yasser Department of Pharmacy - COMSATS University Islamabad - Lahore Campus - Lahore, Pakistan , Khalid, Syed Haroon Department of Pharmaceutics - Faculty of Pharmaceutical Sciences - Government College University Faisalabad, Pakistan , Asghar, Sajid Department of Pharmaceutics - Faculty of Pharmaceutical Sciences - Government College University Faisalabad, Pakistan , Yousaf, Abid Mehmood Department of Pharmacy - COMSATS University Islamabad - Lahore Campus - Lahore, Pakistan , Hussain, Talib Department of Pharmacy - COMSATS University Islamabad - Lahore Campus - Lahore, Pakistan , Irfan, Muhammad Department of Pharmaceutics - Faculty of Pharmaceutical Sciences - Government College University Faisalabad, Pakistan , Khalid, Ikrima Department of Pharmaceutics - Faculty of Pharmaceutical Sciences - Government College University Faisalabad, Pakistan
Abstract :
A simple, specific, accurate and economic reverse phase liquid chromatographic method wasdeveloped forthe estimation of Sofosbuvir in all polymorphic form. Mobile phase containsacetonitrile and 0.05M ammonium acetate (pH 7.6) in ratio of 50:50 (v/v). A logarithmiccalibration curve was plotted from10μgmL-1to60μgmL-1(r= 0.9989) for sofosbuvir, withthelimit of detection (LOD) and the limit of quantification(LOQ)of 0.245μgmL-1and 0.816μgmL-1,respectively. The developed method was highly validated andpracticed for the assurance ofsofosbuvir raw materials with acceptable and non-objectionable accuracy and precision results(recovery 99–102%, RSD <1.2%, n = 3).Zero significant difference (t-test) was obtained betweenthe overall results of the developed RP-HPLC method.
Keywords :
Calibration , Chromatography , Developed , Accuracy , Sofosbuvir
