Molecular Detection and Immune Response of Bee Product Therapy for Induction of Endogenous Mesenchymal Stem Cells of Infertile Male Wistar Rat’s (Rattus norvegicus)

Molecular detection and immune response like HSP70 and PGE2 of bee product therapy has proven can for using as alternative of stem cells therapy of infertile male rat (Rattus norvegicus). The stem cells therapy can cope with various diseases including infertility caused by the degenerative conditions of the testis. Nevertheless, the complexity of the isolation method, in vitro culture process and transplant procedure with booster treatment are very expensive. The innovation was needed as an effort to alternative through of induction of endogenous stem cells. Forty rats were devided 4 groups: Normal rats, without honey (T-); infertile rats, without honey (T+); infertile rats were given 30% honey (v/v) for 10 days (T1) and infertile rats were given 50% honey (v/v) for 10 days (T2). The result, HSP70 and PGE2 expression as a immune response for regenerative signal in group, T-, T+, T1 and T2 respectively is 0.15a ± 0.5; 3.15b ± 0.4; 2.95b ± 0.35 and 0.75a ± 0.15 (for HSP70) and 1.15b ± 0.00; 0.19a ± 0.53; 0.27a ± 0.39 and 2.95c ± 0.45 (for PGE2). Number of Leydig and spermatogenic cells (spermatogonia, spermatocyte primer-seconder and spermatid) in T+ was significantly decreased (p<0.05) when compared with T-, T1 and T2, while T2 didn’t show decreased significantly (p>0.05) compared with T-. It could be concluded, that Molecular detection and immune response of bee product therapy was based on high increase of PGE2 and few increase of HSP70 in testicular tissue and then can rescue of infertile male rat with degenerative testis.