Application of Cloud Point Method for SpectrophotometricDetermination of Salbutamol Sulphate and Methyldopa

A simple and efficient cloud point spectrophotometric method has been used for the determination ofsalbutamol sulphate and methyldopa both in pure and pharmaceutical preparations. The procedure was basedon the ion association formation witheosin Y. The extraction of ion association, drown to Triton X-114micelles, was measured spectrophotometrically. The phase separation wasstudied and optimized. Beer's lawwas rectilinear over the concentration ranges of 0.1-20 and 0.3-10 μg/mLwith molar absorptivity 4x104and5.7x104L.mol-1cm-1and average recovery 98.21% and 101.27% for the above drugs,respectively. Themethod was applied successfully for the determination of salbutamol sulphate and methyldopa inpharmaceuticals.