Title of article
Development of Colorimetric Method for the QuantitativeAnalysis ofAmlodipine Besylate in Dosage Form Using 4-Dimethyleaminobenzaldehydeas Derivatizing Reagent
Author/Authors
Memon, Abdul Ghani University ofSindh - Jamshoro, Pakistan , Memon, Ayaz Ali University ofSindh - Jamshoro, Pakistan , Mahboob AliRind, Faqeer University ofSindh - Jamshoro, Pakistan , Zounr, Zahid Ali University ofSindh - Jamshoro, Pakistan , Pirzada, Azhar AliAyaz Department of Electronic Engineering - University of Sindh - Jamshoro, Pakistan , Brohi, Nazir Ahmed Department of Microbiology - University of Sindh - Jamshoro, Pakistan , Khaskheli, Mazhar Iqbal University ofSindh - Jamshoro, Pakistan , Memon, Jamil Rahman University ofSindh - Jamshoro, Pakistan
Pages
7
From page
27
To page
33
Abstract
The study is based on the determination of amlodipine besylate (AB) after derivatization with 4-dimethylaminobenzaldehyde (DMAB) using UV/Visiblespectrophotometer at pH 5 to yieldderivative that is measured atλmax399nm.ThecalibrationgraphobtainedwaslinerandfulfilledBeerLambert’s law in the concentration range 05-25 μg/mL of (AB) and DMAB havingcoefficient of determination R² 0.9988 with RSD0.93%and molar absorptivity 4.04x103mole-1cm-1. Quantitative /analytical parameters such as pH, heating time, temperature were optimized.Reagent concentration /volume, interday and intraday studies were also carried out. There was noeffect of various solvents and additivesobservedon the determination ofABin commerciallyavailable drugs. The method is stable, accurate, rapid and simple for the study of imine derivativeofAB.
Keywords
Colorimetric method , Amlodipine besylate , 4-dimethyleaminobenzaldehyde , Derivatization
Journal title
Pakistan Journal of Analytical and Environmental Chemistry
Serial Year
2020
Full Text URL
Record number
2596279
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