Author/Authors :
Memon, Abdul Ghani University ofSindh - Jamshoro, Pakistan , Memon, Ayaz Ali University ofSindh - Jamshoro, Pakistan , Mahboob AliRind, Faqeer University ofSindh - Jamshoro, Pakistan , Zounr, Zahid Ali University ofSindh - Jamshoro, Pakistan , Pirzada, Azhar AliAyaz Department of Electronic Engineering - University of Sindh - Jamshoro, Pakistan , Brohi, Nazir Ahmed Department of Microbiology - University of Sindh - Jamshoro, Pakistan , Khaskheli, Mazhar Iqbal University ofSindh - Jamshoro, Pakistan , Memon, Jamil Rahman University ofSindh - Jamshoro, Pakistan
Abstract :
The study is based on the determination of amlodipine besylate (AB) after derivatization with 4-dimethylaminobenzaldehyde (DMAB) using UV/Visiblespectrophotometer at pH 5 to yieldderivative that is measured atλmax399nm.ThecalibrationgraphobtainedwaslinerandfulfilledBeerLambert’s law in the concentration range 05-25 μg/mL of (AB) and DMAB havingcoefficient of determination R² 0.9988 with RSD0.93%and molar absorptivity 4.04x103mole-1cm-1. Quantitative /analytical parameters such as pH, heating time, temperature were optimized.Reagent concentration /volume, interday and intraday studies were also carried out. There was noeffect of various solvents and additivesobservedon the determination ofABin commerciallyavailable drugs. The method is stable, accurate, rapid and simple for the study of imine derivativeofAB.
Keywords :
Colorimetric method , Amlodipine besylate , 4-dimethyleaminobenzaldehyde , Derivatization
