Evaluation of Antibacterial Activity of Zinc OxideNanoparticles and Acrylamide Composite AgainstMultidrug-Resistant PathogenicBacteria

Emerging antibiotic resistancein pathogenic bacteria is creating serious crises in therapeuticoptions for treating infections worldwide. Thus, in the quest of alternative efficacious antibacterialtherapy, various previous studies have demonstrated that the coating material used forthesynthesis of Zinc oxide nanoparticles has tremendously improved the antibacterial activity ofnanoparticles. The aim of current study was to investigate the antibacterial activities of Zinc oxidenanoparticles and acrylamide composite (ZnO-Am-NPs) against multidrug-resistant pathogenicbacteria. Isolation and identification was performed by using standard conventional andbiochemical techniques. The antimicrobial activity of ZnO-Am-NPs was determined by usingmodified agar well diffusion assay. The efficacy of ZnO-Am-NPs was compared withcommerciallyavailable standard antibiotics discs. The data showed that ZnO-Am-NPs possessedstrong antibacterial activity against multidrug-resistant pathogenic bacteria includingEscherichiacoli,Pseudomonas aeruginosa, Klebsiella pneumoniae,andStaphylococcus aureus, suggestingthat coating of ZnO-NPs with acrylamide resulted in the broad spectrum antibacterial activity. Theantibacterial activity increased with the increasing concentration of ZnO-Am-NPs whereas theminimum inhibitory concentration of ZnO-Am-NPs was recorded as 12.5μg/ml. The results ofpresent study indicated that the ZnO-Am-NPs may serve as promising antibacterial agents againstmultidrug resistant and medically important bacteria.