Development and Validation of HPLC Method for theSimultaneous Determination of Insecticides (Carbofuranand Fipronil) in its Technicaland Granular Formulation

A precise, accurate, simple and low costreversephase high performanceliquid chromatographic(RP-HPLC) method was developed and validated for the simultaneous quality assessment ofcommonly used insecticide carbofuran and fipronil in its technical and formulated products. Thechromatographic parameters, i.e. mobile phase composition, wavelength and flow rate ofdeveloped method was optimized in such a way that the HPLC was carried out at 280 nm by usinga mobile phase, which contains 80 parts acetonitrile and 20 parts water by volume (ACN:H2O,80:20) flowing at a rate of 1.0 mL/min. A linear relationship (R2> 0.999) was shown bycalibration curve plotted in the concentration ranged from 30-800 mg/L. Thelimit of detection(LODs)were found to be 14.5 mg/L and 20.9 mg/L andlimit of quantification (LOQs)wererecorded to be 43.9 mg/L and 63.2 mg/L for both carbofuran and fipronil respectively. Thedeveloped method’s accuracy was exhibited by recovery percentage (96.0-100.8) % of watersamples spiked with 100, 300 and 600 mg/Lof carbofuran and fipronil,respectively. Thedeveloped RP-HPLC method is therefore simple, cheap, rapid and can be proficiently used for thesimultaneous identification and quantification of carbofuran and fipronil in its technical andformulated products.