Examination of Impact of Arsenic Stress on Soil Microbes

The research was performed at Botany department, Nusrat Jahan College Rabwah Pakistan toscreen stress tolerance level of soil microbes (pseudomonas and penicillium) taken from NARC(National Agricultural Research Centre)Pakistanagainst different levels of sodium arsenitestress (1mg/Lto10mg/L). The research was based on completely randomized design. Threedrops were applied to microbes grown on CLED (cysteine-, lactose-, and electrolyte-deficient)media to determine zone of inhibition through disk, well and spread method, while control groupwas without sodium arsenite application. After application of various levels of arsenic stressorganisms wereincubated for 24 h at 37°C. After incubation, zones of inhibition were measured.Our study has shown that both micobes cannot overcome higher levels of arsenic stress because inhigher stress petriplates, increased inhibitory zones were observed.