Molecular study of Trypanosoma spp in camels of Al- Diwaniyah province in Iraq

Due to the economic importance of the camels and because of the scarcity of studies that death with it in Iraq, our study aimed to investigate Trypanosoma spp by molecular methods. The current study was conducted during the period from September 2017 to the month March 2018 and a total of 200 samples were collected randomly from camels included (125( females and )75( males and two groups of ages )150( sample 1 years and)50( sample 1year ) some of them have clinical signs and some did not show any symptoms from the slaughterhouse of Al- Diwaniya province. This study was designed to diagnose this parasite were initially diagnosed by microscopic examination and was examined by geimesa stain method and the results were positive of (76/200) (38%) Trypanosoma spp. The prevalence of Trypanosomiasis in females (57/125) (45.6%) while males (19/75) (25.3%) and in all ages. The highest incidence in months (September, October and March) (88.3%, 96.2% and 75%), respectively. Secondly the diagnosis of Trypanosoma in 50 blood samples positive microscopically using molecular techniques, which included polymerase chain reaction (conventional PCR) .The results (10/50)(20%) Trpanosoma spp and (7/50)(14%) T. evansi then DNA sequencing analysis and detecting genetic relationships (phylogenic analysis).