Detection of H.pylori cagA gene in patient s with gastroduodenal disease

Helicobacter pylori infection is common in the developing countries. The cagA gene is a marker of pathogenicity island (PAI) in H. pylori. The aim of this study was to determine the prevalence of cagA among gastroduodenal disease (peptic ulcer and non-ulcer dyspepsia) in patients of Ramadi- city. A total of 81 gastric biopsy samples, gastric ulcer 10 (12.30% ) , duodenal ulcer 18 (22.20% ) and non-ulcer dyspepsia 53 (65.40% ) by endoscopic examination . cagA gene of H. pylori was assessed using polymerase chain reaction (PCR). The cagA gene was detected by PCR technique in 3 from 10 biopsy specimens culture positive, urease test was showed 7(8.64%) patients, 2(2.5%) patients and 48(59.26%)patients after (2 hr, 6hr and 20 hr) respectively, ELISA positive result was showed 12(14.8%) patients. Urease test is easy test , inexpensive and can be use in endoscopy unit, but non-specific, culture is gold stander for detection of H.pylori, ELISA technique use for detection of anti-H.pylori IgG , ELISA technique widly used for epidemiology studies. PCR assay used for detection of cagA gene. Using PCR to detect cagA gene from culture colony. The potential advantages of PCR include high specificity, quick results and the ability to type bacteria without the requirement for special transport conditions..