Detection of genotypes for Giardia lamblia in Iraqi patients feces by using PCR-RFLP techniques based on GDH gene characterization

This study was carried out during the period from March 2016 to January 2017 to detect of genotypes and subgenotypes of Giardia lamblia in human feces by PCR-Restriction fragment length polymorphism based on the detection of glutamate dehydrogenase gene and study the corralation between genotype and prescence of symptoms , a total of 101 stool samples were taken from patients(male and female), aged(2-72) years, who suffering from acute or persistent diarrhea and examined by microscope and molecular techniques , the gdh gene was amplified from 19 cases only by a conventional PCR assay by using a specific oligonucleotide sequence for coding region by gdh gene at size 432bp and typed by RFLP analysis, the results showed the total infection with Giardia lamblia was 84(83.1%) according to the results of microscopic examination, of the 19 positive samples, 2(1.98%) were infected with genotype AI , 3(2.97%) genotype AII , 5(4.95%) genotype BIII and 9(8.91%) infected with genotype BIV, with significance differences between the genotype and precence of symptoms.