Validation of Rapid Gas Chromatographic Method for Determination of Seven Volatile Compounds in a Urinary Tract Antiseptic Soft Gelatin Capsules

A simple, rapid and precise gas chromatographic method has been developed for simultaneous determination of seven volatile compounds namely alpha pinene, camphene, beta pinene, cineole, fenchone, borneol and anethol in urinary tract antiseptic soft gelatine capsule. The seven compounds were analysed by Gas chromatography-flame ionization detector (GC-FID) on a (5% diphenyl and 95% dimethylpolysiloxane) (30m x0.25mm x 0.25μm) column and Helium as a carrier gas. The injector and detector port temperatures were maintained at 200°C and 250°C respectively. Results of assay and recovery studies were statistically evaluated for its accuracy and precision. The correlation coefficient (r) values ranged from 0.997 to 0.9998. The detection limits ranged from 0.0015 to 0.014 mg ml^−1. No interference from any components of pharmaceutical dosage forms was observed. According to the validation results, the proposed method was found to be specific, accurate and precise and could be applied to the simultaneous quantitative analysis of these seven volatile compounds in such pharmaceutical formulation.