Author/Authors :
Shakir, I . M.A. University of Baghdad - College of Science - Department of Chemistry, Iraq , Turkie, N. S. University of Baghdad - College of Science - Department of Chemistry, Iraq , Ulaiwi, W. S. University of Baghdad - College of Agriculture, Iraq
Abstract :
Histidine was determined via measurement of total luminescence (i:e creation of chemiluminescence and insitu irradiation of released light to an acceptor fluorophore molecule to initiate fluorescence from fluorescien molecule in flat – sp iral micro cell designed for this measurement . A d etailed description of robust linear equation for the range of 0.002 – 0.05 mol.L^-1 for a sample size of 70 μL with a correlation coefficient of 0.9879 and a coefficient of determination of 97.59% while for a quadratic model of the same concentration range was 0.9881 correlation coefficient and 97.63% coefficient of determination. Analysis of variance was conducted for both kinds of models . It indicated that their was no significant difference and both equations can be used to represent the data obtained
