Author/Authors :
Glassmann, Alexander Life Science Inkubator, Bonn, Germany , Carrillo Garcia, Carmen Department of Internal Medicine III - Division of Hematology and Oncology - University of Bonn, Bonn, Germany , Janzen, Viktor Department of Internal Medicine III - Division of Hematology and Oncology - University of Bonn, Bonn, Germany , Kraus, Dominik Department of Prosthodontics - Preclinical Education, and Material Science - University of Bonn, Bonn, Germany , Veit, Nadine Neuro- and Tumor Cell Biology Group - Department of Nuclear Medicine - University of Bonn, Bonn, Germany , Winter, Jochen Oral Cell Biology Group - Department of Periodontology - Operative and Preventive Dentistry - University of Bonn, Bonn, Germany , Probstmeier, Rainer Neuro- and Tumor Cell Biology Group - Department of Nuclear Medicine - University of Bonn, Bonn, Germany
Abstract :
Cultivation of A549 non-small-cell lung carcinoma (NSCLC) cells in the presence of staurosporine (SSP) leads to a reduction or alack of proliferation in a concentration-dependent manner. This inhibition of proliferation is accompanied by the generation ofpolyploid giant cancer cells (PGCCs) that are characterized by cellflattening, increased cell size, polyploidy, and polynucleationas determined by crystal violet staining, BrdU and DiI labelling, andflow cytometry as well as video time-lapse analysis.Continuous SSP treatment of A549 cells can preserve PGCCs for at least two months in a resting state. Upon removal of SSP,A549 PGCCs restart to divide and exhibit a proliferation pattern and cellular morphology indistinguishable from cells wherePGCCs originally derived from. Thus, SSP-treated A549 cells represent a simple and reliable experimental model for thereversible generation of PGCCs and their subsequent experimental analysis.
