Title of article :
GTSE1, CDC20, PCNA, and MCM6 Synergistically AffectRegulations in Cell Cycle and Indicate Poor Prognosis inLiver Cancer
Author/Authors :
Zheng, Yongchang Department of Liver Surgery - Peking union Medical College Hospital - Chinese Academy of Medical Sciences & Peking unionMedical College, Beijing, China , Shi, Yue Department of Liver Surgery - Peking union Medical College Hospital - Chinese Academy of Medical Sciences & Peking unionMedical College, Beijing, China , Yu, Si Department of Liver Surgery - Peking union Medical College Hospital - Chinese Academy of Medical Sciences & Peking unionMedical College, Beijing, China , Han, Yuanyuan Center of Tree Shrew Germplasm Resources - Institute of Medical Biology - The Chinese Academy of Medical Sciences and Pekingunion Medical College - Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Kunming,Yunnan, China , Kang, Kai Department of Liver Surgery - Peking union Medical College Hospital - Chinese Academy of Medical Sciences & Peking unionMedical College, Beijing, China , Xu, Haifeng Department of Liver Surgery - Peking union Medical College Hospital - Chinese Academy of Medical Sciences & Peking unionMedical College, Beijing, China , Gu, Huajian Department of Hepatobiliary Surgery - Affiliated Hospital of Guizhou Medical University, Guiyang, China , Sang, Xinting Department of Liver Surgery - Peking union Medical College Hospital - Chinese Academy of Medical Sciences & Peking unionMedical College, Beijing, China , Chen, Yang MOE Key Laboratory of Bioinformatics - Center for Synthetic and Systems Biology - Bioinformatics Division - BNRist,Department of Automation - Tsinghua University, Beijing, China , Wang, Jingyu MOE Key Laboratory of Bioinformatics - Center for Synthetic and Systems Biology - Bioinformatics Division - BNRist,Department of Automation - Tsinghua University, Beijing, China
Abstract :
GTSE1 is well correlated with tumor progression; however, little is known regarding its role in liver cancer prognosis. By analyzingthe hepatocellular carcinoma (HCC) datasets in GEO and TCGA databases, we showed that high expression of GTSE1 wascorrelated with advanced pathologic stage and poor prognosis of HCC patients. To investigate underlying molecularmechanism, we generated GTSE1 knockdown HCC cell line and explored the effects of GTSE1 deficiency in cell growth.Between GTSE1 knockdown and wild-type HCC cells, we identified 979 differentially expressed genes (520 downregulated and459 upregulated genes) in the analysis of microarray-based gene expression profiling. Functional enrichment analysis of DEGssuggested that S phase was dysregulated without GTSE1 expression, which was further verified fromflow cytometry analysis.Moreover, three other DEGs: CDC20, PCNA, and MCM6, were also found contributing to GTSE1-related cell cycle arrest andto be associated with poor overall survival of HCC patients. In conclusion, GTSE1, together with CDC20, PCNA, and MCM6,may synergistically promote adverse prognosis in HCC by activating cell cycle. Genes like GTSE1, CDC20, PCNA, and MCM6may be promising prognostic molecular biomarkers in liver cancer.
Keywords :
Liver Cancer , Cell Cycle , Poor Prognosis , MCM6 Synergistically
