Author/Authors :
Lu, Kang Department of Orthopedics/Sports Medicine Center - State Key Laboratory of Trauma - Burn and Combined Injury,Southwest Hospital - Army Medical University (Third Military Medical University), Chongqing, China , Chen, Xiaodie institute for Clean Energy & Advanced Materials - School of Materials & Energy - Southwest University, Chongqing, China , Tang, Hong Department of Orthopedics/Sports Medicine Center - State Key Laboratory of Trauma - Burn and Combined Injury,Southwest Hospital - Army Medical University (Third Military Medical University), Chongqing, China , Zhou, Mei Department of Orthopedics/Sports Medicine Center - State Key Laboratory of Trauma - Burn and Combined Injury,Southwest Hospital - Army Medical University (Third Military Medical University), Chongqing, China , He, Gang Department of Orthopedics/Sports Medicine Center - State Key Laboratory of Trauma - Burn and Combined Injury,Southwest Hospital - Army Medical University (Third Military Medical University), Chongqing, China , Liu, Juan Department of Orthopedics/Sports Medicine Center - State Key Laboratory of Trauma - Burn and Combined Injury,Southwest Hospital - Army Medical University (Third Military Medical University), Chongqing, China , Bian, Xuting Department of Orthopedics/Sports Medicine Center - State Key Laboratory of Trauma - Burn and Combined Injury,Southwest Hospital - Army Medical University (Third Military Medical University), Chongqing, China , Guo, Yupeng Department of Orthopedics/Sports Medicine Center - State Key Laboratory of Trauma - Burn and Combined Injury,Southwest Hospital - Army Medical University (Third Military Medical University), Chongqing, China , Lai, Fan Department of Orthopedics/Sports Medicine Center - State Key Laboratory of Trauma - Burn and Combined Injury,Southwest Hospital - Army Medical University (Third Military Medical University), Chongqing, China , Yang, Mingyu Department of Orthopedics/Sports Medicine Center - State Key Laboratory of Trauma - Burn and Combined Injury,Southwest Hospital - Army Medical University (Third Military Medical University), Chongqing, China , Lu, Zhisong Department of Orthopedics/Sports Medicine Center - State Key Laboratory of Trauma - Burn and Combined Injury,Southwest Hospital - Army Medical University (Third Military Medical University), Chongqing, China , Tang, Kanglai Department of Orthopedics/Sports Medicine Center - State Key Laboratory of Trauma - Burn and Combined Injury,Southwest Hospital - Army Medical University (Third Military Medical University), Chongqing, China
Abstract :
Purpose. Tendon injuries are common musculoskeletal system disorders, but the ability for tendon regeneration is limited. Silkfibroin (SF)film may be suitable for tendon regeneration due to its excellent biocompatibility and physical properties. This study is aimed at evaluating the application value of bionic SFfilm in tendon regeneration.Methods. Tendon stem/progenitorcells (TSPCs) were isolated from rat Achilles tendon and characterized based on their surface marker expression andmultilineage differentiation potential. SFfilms with smooth or bionic micro structure surfaces (5, 10, 15, 20μm) were prepared.The morphology and mechanical properties of natural tendons and SF films were characterized. TSPCs were used as the seed cells, and the cell viability and cell adhesion morphology were analyzed. The tendongenesis-related gene expression of TSPCswas also evaluated using quantitative polymerase chain reaction.Results. Compared to the native tendon, only the 10, 15, and20μmSFfilm groups had comparable maximum loading and ultimate stress, with the exception of the breaking elongation rate.The 10μmSFfilm group had the highest percentage of oriented cells and the most significant changes in cell morphology. Themost significant upregulations in the expression ofCOL1A1,TNC,TNMD,andSCXwere also observed in the 10μmSFfilmgroup.Conclusion.SFfilm with a bionic microstructure can serve as a tissue engineering scaffold and provide biophysical cuesfor the use of TSPCs to achieve proper cellular adherence arrangement and morphology as well as promote the tenogenicdifferentiation of TSPCs, making it a valuable customizable biomaterial for future applications in tendon repair.
