Author/Authors :
Wang, Wen-Jun Department of Respiratory and Critical Care Medicine - Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences)- Guangdong Provincial Geriatrics Institute - First Clinical Medicine Institute of South China University of Technology, Guangzhou, Guangdong, China , Yang, Shi-Fang Department of Respiratory and Critical Care Medicine - Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences)- Guangdong Provincial Geriatrics Institute - First Clinical Medicine Institute of South China University of Technology, Guangzhou, Guangdong, China , Gao, Zhi-Rui Department of Respiratory and Critical Care Medicine - Yan’an Hospital of Kunming, Kunming, China , Luo, Ze-Ru Department of Respiratory and Critical Care Medicine - Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences)- Guangdong Provincial Geriatrics Institute - First Clinical Medicine Institute of South China University of Technology, Guangzhou, Guangdong, China , Liu, Yuan-Ling Department of Respiratory and Critical Care Medicine - Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences)- Guangdong Provincial Geriatrics Institute - First Clinical Medicine Institute of South China University of Technology, Guangzhou, Guangdong, China , Gao, Xing-Lin Department of Respiratory and Critical Care Medicine - Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences)- Guangdong Provincial Geriatrics Institute - First Clinical Medicine Institute of South China University of Technology, Guangzhou, Guangdong, China
Abstract :
Introduction. Some studies have found that cilia were shorter in COPD smokers than in nonsmokers or healthy smokers.
However, the structural abnormalities of cilia and the cause of such abnormalities in COPD patients still remain unknown. Tumor
necrosis factor alpha receptor 3 interacting protein 1 (MIP-T3) may play an important role in the progress of ciliary protein
transporting. Objectives. ,is study aimed at exploring the dominated structural abnormalities of cilia and the involvement of
MIP-T3 in the pathogenesis of cilia of COPD patients. Methods. Patients who accepted pulmonary lobectomy were divided into 3
groups: the chronic obstructive pulmonary disease (COPD) smoker group, the healthy smoker group, and the nonsmoker group,
according to smoking history and pulmonary function. The ultrastructure of cilia and the percentage of abnormal cilia were
analyzed using a transmission electron microscope. Real-time PCR, immunohistochemical staining, and western blotting in
bronchial epithelium were used to determine MIP-T3 mRNA and protein expression. The relationship between the percentage of
abnormal cilia and lung function and MIP-T3 protein expression was analyzed. Results. Patients in the COPD smoker group had
increased percentage of abnormal cilia comparing to both the healthy smoker group and the nonsmoker group (both P values
<0.05). MIP-T3 expression was significantly declined in the COPD smoker group (P value <0.05). Moreover, the percentage of
abnormal cilia was negatively correlated with FEV1, FEV1/FVC ratio, and FEV1%pred (all P values <0.05). Moreover, the MIP-T3
protein expression was positively correlated with the percentage of abnormal cilia (P value <0.05). Conclusions. Our results
suggested that the abnormal ciliary ultrastructure, which was common in COPD patients, might be due to MIPT3 downregulation.
Keywords :
COPD Patients , Airway , MIP-T3 Expression , Ciliogenesis
