Title of article :
The Effect of Piperine on MMP-9, VEGF, and E-cadherin Expression in Breast Cancer MCF-7 Cell Line
Author/Authors :
Zare ، Zahra Department of Biology - Farhangian University , Nayerpour Dizaj ، Tina Department of Medical Biotechnology - Faculty of Modern Sciences - Tabriz University of Medical Sciences , Lohrasbi ، Armaghan Department of Biological and Biomedical Sciences - Glasgow Caledonian University , Sheikhalishahi ، Zakieh Sadat Department of Physiology - Shahid Sadoghi University of Medical Sciences , Panji ، Mohammad Department of Cell and Molecular Biology - Faculty of Biological Science - Kharazmi University , Hosseinabadi ، Fahimeh Biology Department - Faculty of Sciences - Arak University , Najafi ، Vajiheh Research Center for Biochemistry and Nutrition in Metabolic Diseases - Kashan University of Medical Sciences , Abazari ، Omid Department of Clinical Biochemistry - School of Medicine - Shahid Sadoughi University of Medical Sciences and Health Services , Abbasi ، Mojtaba Faculty of Veterinary Medicine - Islamic Azad University, Shahrekord Branch , Khanicheragh ، Parisa Department of Clinical Biochemistry - Lorestan University of Medical Sciences
Abstract :
Background: Vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9), and E-cadherin play a vital role in the behavior of angiogenesis, metastasis, and invasion of breast tumor cells. Piperine, the main component of Piper Nigrum, has shown anti-cancer properties in various malignancies. This Study investigates the potential effect of piperine on MMP-9, E-cadherin, and VEGF expression in breast cancer MCF-7 cell line. Methods: MTT assay was applied to assess the viability of MCF-7 cells. The mRNA levels of MMP-9, VEGF, and E-cadherin were assayed by qRT-PCR. Western blot was performed to identify the protein level of MMP-9. Results: MTT assay results showed that piperine treatment (5, 10, 25, 50, 75, and 100 μM) for 24 hours effectively inhibited cell viability of MCF-7 cells as compared with the control group. Furthermore, the gene expression of VEGF, MMP-9, and E-cadherin was dose-dependently suppressed by piperine treatment (5, 10 and 25 μM) (P 0.05; P 0.01). The results also indicated that piperine (5, 10, and 25 μM) significantly suppressed MMP-9 protein expression after 24 hours of piperine treatment (P 0.01). Conclusion: These results suggest that piperine may prevent angiogenesis, migration, and invasion of MCF-7 cells by suppressing MMP-9 and VEGF, and by inducing E-cadherin expression. Hence, it may be a suitable candidate for designing new drugs in cancer therapy.
Keywords :
Piperine , MCF , 7 , MMP , 9 , VEGF , E , cadherin
Journal title :
Basic and Clinical Cancer Research
Journal title :
Basic and Clinical Cancer Research
