Title of article :
Origanum majorana Essential Oil Lacks Mutagenic Activity in the Salmonella/Microsome and Micronucleus Assays
Author/Authors :
dos Santos Dantas, Andrea Programa de Pos-Graduac ´ ¸ao em Ci ˜ encias Veterin ˆ arias - UFRGS - Porto Alegre - RS - Brazil ´ - Laboratorio de Produtos Naturais/Fitoqu ´ ´ımica-Farmacologia e Toxicologia Veterinaria - Departamento de Farmacologia - ´ UFRGS - Porto Alegre - RS - Brazil , Klein-Júnior, Luiz Carlos Laboratorio de Farmacognosia e Controle de Qualidade de Fitomedicamentos - Faculdade de Farm ´ acia - UFRGS - ´ Porto Alegre - RS - Brazil , Machado, Miriana S Laboratorio de Reparac ´ ¸ao de DNA em Eucariotos - Departamento de Biof ˜ ´ısica/Centro de Biotecnologia - UFRGS - Porto Alegre - RS - Brazil , Guecheva, Temenouga N Laboratorio de Reparac ´ ¸ao de DNA em Eucariotos - Departamento de Biof ˜ ´ısica/Centro de Biotecnologia - UFRGS - Porto Alegre - RS - Brazil , dos Santos, Luciana D Programa de Pos-Graduac ´ ¸ao em Ci ˜ encias Veterin ˆ arias - UFRGS - Porto Alegre - RS - Brazil ´ - Laboratorio de Produtos Naturais/Fitoqu ´ ´ımica-Farmacologia e Toxicologia Veterinaria - Departamento de Farmacologia - ´ UFRGS - Porto Alegre - RS - Brazil , Zanette, Régis A Programa de Pos-Graduac ´ ¸ao em Farmacologia e Terap ˜ eutica - UFRGS - Porto Alegre - RS - Brazil , de Mello, Fernanda B Laboratorio de Produtos Naturais/Fitoqu ´ ´ımica-Farmacologia e Toxicologia Veterinaria - Departamento de Farmacologia - ´ UFRGS - Porto Alegre - RS - Brazil , Pêgas Henriques, João Antonio Laboratorio de Reparac ´ ¸ao de DNA em Eucariotos - Departamento de Biof ˜ ´ısica/Centro de Biotecnologia - UFRGS - Porto Alegre - RS - Brazil , Braga de Mello, João Roberto Programa de Pos-Graduac ´ ¸ao em Ci ˜ encias Veterin ˆ arias - UFRGS - Porto Alegre - RS - Brazil ´ - Laboratorio de Produtos Naturais/Fitoqu ´ ´ımica-Farmacologia e Toxicologia Veterinaria - Departamento de Farmacologia - ´ UFRGS - Porto Alegre - RS - Brazil
Abstract :
The present study aimed to investigate the in vitro mutagenic activity of Origanum majorana essential oil. The most abundant compounds identified by GC-MS were 𝛾-terpinene (25.73%), 𝛼-terpinene (17.35%), terpinen-4-ol (17.24%), and sabinene (10.8%).
Mutagenicity was evaluated by the Salmonella/microsome test using the preincubation procedure on TA98, TA97a, TA100, TA102, and TA1535 Salmonella typhimurium strains, in the absence or in the presence of metabolic activation. Cytotoxicity was detected at
concentrations higher than 0.04 𝜇L/plate in the absence of S9 mix and higher than 0.08 𝜇L/plate in the presence of S9 mix and no
gene mutation increase was observed. For the in vitro mammalian cell micronucleus test, V79 Chinese hamster lung fibroblasts were
used. Cytotoxicity was only observed at concentrations higher than or equal to 0.05 𝜇g/mL. Moreover, when tested in noncytotoxic concentrations, O. majorana essential oil was not able to induce chromosome mutation.The results from this study therefore suggest that O. majorana essential oil is not mutagenic at the concentrations tested in the Salmonella/microsome and micronucleus assays.
Keywords :
Origanum majorana Essential , Oil Lacks Mutagenic Activity , Salmonella/Microsome , Micronucleus Assays
Journal title :
The Scientific World Journal
