Title of article :
Somatic Mutations in Exons 10, 11, and 16 of the RET Protooncogene in Medullary Thyroid Carcinoma Patients
Author/Authors :
Shakiba, Elham Department of Cellular and Molecular Biology - Faculty of Biological Sciences - North Tehran Branch - Islamic Azad University, Tehran, Iran , Hedayati, Mehdi Cellular and Molecular Endocrine Research Center - Research Institute for Endocrine Sciences - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Majd, Ahmad Department of Cellular and Molecular Biology - Faculty of Biological Sciences - North Tehran Branch - Islamic Azad University, Tehran, Iran , Movahedi, Monireh Department of Cellular and Molecular Biology - Faculty of Biological Sciences - North Tehran Branch - Islamic Azad University, Tehran, Iran
Abstract :
Introduction: Medullary thyroid carcinoma (MTC) comprises nearly 5% of all cases of thyroid cancer
(TC). Aberrant activation of RET (rearranged during transfection) signaling via somatic mutations is
the basic molecular mechanism of MTC tumorigenicity. In this study, we determined the incidence of
RET gene mutations in exons 10, 11, and 16 in Iranian patients.
Methods: A total of 33 patients undergoing thyroidectomy at Imam Khomeini hospital of Tehran, Iran
and diagnosed with MTC were enrolled. For investigating mutations in exons 10, 11, and 16, DNA was
extracted from tumor tissues, and the genes were amplified by polymerase chain reaction (PCR) and
then sequenced.
Results: Out of 33 patients, 20 (60.6%) subjects had mutations in one of the examined exons (10, 11,
and 16). According to our results, the “ATG918ACG” mutation in codon 918 had the highest rate.
Conclusion: Testing RET mutations can be beneficial in clinical evaluation and treatment management
of MTC patients.
Farsi abstract :
فاقد چكيده فارسي
Keywords :
Thyroid , Caner , Medullary thyroid carcinoma , RET gene , Somatic mutations
Journal title :
International Journal of Basic Science in Medicine (IJBSM)
