Author/Authors :
Zou, Kejian Department of General Surgery - Hainan General Hospital, Haikou, China , Hu, Yan Department of Anesthesiology - Hainan General Hospital, Haikou, China , Li, Musheng Department of Gastroenterology - Guangzhou Women and Children’s Medical Center - Guangzhou Medical University, Guangzhou, China , Wang, Hongli Department of Gastroenterology - Guangzhou Women and Children’s Medical Center - Guangzhou Medical University, Guangzhou, China , Zhang, Yuhua Department of Pediatrics - Te Ninety-Five Hospital of PLA, Putian, China , Huang, Ling Department of Gastroenterology - Guangzhou Women and Children’s Medical Center - Guangzhou Medical University, Guangzhou, China , Xie, Yuanwen Department of Anorectal - Qionghai Hospital of Traditional Chinese Medicine, Qionghai, China , Li, Songyu Department of Clinical Laboratory - Qionghai Hospital of Traditional Chinese Medicine, Qionghai, China , Dai, Xingui Department of Critical Care Medicine - Institute of Transitional Medicine - Te First People’s Hospital of Chenzhou, Chenzhou, China , Xu, Wanfu Department of Gastroenterology - Guangzhou Women and Children’s Medical Center - Guangzhou Medical University, Guangzhou, China , Ke, Zhiyong Department of Cell Biology - School of Basic Medical - Southern Medical University, Guangzhou, China , Gong, Sitang Department of Gastroenterology - Guangzhou Women and Children’s Medical Center - Guangzhou Medical University, Guangzhou, China , Wang, Yaodong Kunshan Afliated Hospital of Nanjing University of Chinese Medicine, Kunshan, China
Abstract :
Objective
HMGCS2 is the rate-limiting enzyme of ketogenesis, which is vital for tumor initiation or metastasis. The aim of this study is to determine the relationship between HMGCS2 and tumor angiogenesis.
Materials and Methods
The study consisted of 100 cases with colorectal cancer and healthy control, the expression of HMGCS2 and the microvessel density (MVD) (marker: CD31) were analyzed by immunohistochemistry and tube formation, and the centration of β-hydroxybutyrate in serum was assessed by biochemical analysis.
Results
The results showed that HMGCS2 expression is significantly reduced in colorectal cancer compared with healthy control, which is inversely correlated with MVD in colorectal cancer by IHC analysis. What is more, knockdown HMGCS2 expression in HT-29 cells significantly contributed endothelial cell tube formation.
Conclusion
These findings implying HMGCS2 may have a negative regulation of tumor angiogenesis and provide an approach to inhibit tumor angiogenesis.
