Title of article :
Assessment of Cytotoxic Activity of Rosemary (Rosmarinus officinalis L.), Turmeric (Curcuma longa L.), and Ginger (Zingiber officinale R.) Essential Oils in Cervical Cancer Cells (HeLa)
Author/Authors :
Santos, P. A. S. R Laboratory of Toxicology - Department of Basic Health Sciences - State University of Maringa - Maring ´ a - PR - Brazil , Avanço, G. B Laboratory of Toxicology - Department of Basic Health Sciences - State University of Maringa - Maring ´ a - PR - Brazil , Nerilo, S. B University Center Inga (UNINGA) - Maring ´ a - PR - Brazil , Marcelino, R. I. A Laboratory of Pharmacology and Cellular Toxicology - LFTC/FARMATEC - School of Pharmacy - Federal University of Goias - ´ Goiania - GO - Brazil , Janeiro, V Department of Statistics - State University of Maringa - Maring ´ a - PR - Brazil , Valadares, M. C Laboratory of Pharmacology and Cellular Toxicology - LFTC/FARMATEC - School of Pharmacy - Federal University of Goias - ´ Goiania - GO - Brazil , Machinski, Miguel Laboratory of Toxicology - Department of Basic Health Sciences - State University of Maringa - Maring ´ a - PR - Brazil
Abstract :
The objective of this study was to evaluate the cytotoxic activity of rosemary (REO, Rosmarinus officinalis L.), turmeric (CEO, Curcuma longa L.), and ginger (GEO, Zingiber officinale R.) essential oils in HeLa cells. Cytotoxicity tests were performed in vitro, using tetrazolium (MTT) and neutral red assays for evaluation of antiproliferative activity by different mechanisms, trypan blue
assay to assess cell viability and evaluation of cell morphology for Giemsa to observe the cell damage, and Annexin V to evaluate cell death by apoptosis. CEO and GEO exhibited potent cytotoxic activity against HeLa cells. IC50 obtained was 36.6 𝜇g/mL for CEO and 129.9 𝜇g/mL for GEO. The morphology of HeLa cells showed condensation of chromatin, loss of cell membrane integrity
with protrusions (blebs), and cell content leakage for cells treated with CEO and GEO, from the lowest concentrations studied, 32.81 𝜇g/mL of CEO and 32.12 𝜇g/mL of GEO. The Annexin V assay revealed a profile of cell death by apoptosis for both CEO and GEO. The results indicate cytotoxic activity in vitro for CEO and GEO, suggesting potential use as anticancer agents for cervical cancer cells.
Keywords :
Cytotoxic Activity , Rosemary , Rosmarinus officinalis L. , Turmeric , Curcuma longa L. , Ginger , Zingiber officinale R. , Essential Oils , Cancer Cells , HeLa
Journal title :
The Scientific World Journal
