Title of article :
Leukemia Inhibitory Factor Suppresses NKG2D mRNA Expression and Presentation on Human Natural Killer Cells
Author/Authors :
Abdoli ، Zahra Department of Immunology - School of Medicine, Cancer Research Center - Ahvaz Jundishapur University of Medical Sciences , Assarehzadegan ، Mohammad-Ali Immunology Research Center, Institute of Immunology and Infectious Diseases - Iran University of Medical Sciences , Pipelzadeh ، Mohammad Hassan Department of Pharmacology - Toxicology Research Center, School of Pharmacy - Ahvaz Jundishapur University of Medical Sciences , Iranparast ، Sara Department of Immunology - School of Medicine, Cancer Research Center, Student Research Committee - Ahvaz Jundishapur University of Medical Sciences , Dashti Gerdabi ، Nader Department of Immunology - School of Medicine - Ahvaz Jundishapur University of Medical Sciences , Parsanahad ، Mehdi Department of Virology - School of Medicine - Ahvaz Jundishapur University of Medical Sciences , Khodadadi ، Ali Department of Immunology - School of Medicine, Cancer Research Center - Ahvaz Jundishapur University of Medical Sciences
Abstract :
Leukemia inhibitory factor (LIF) is a multi-functional cytokine secreted from cells such as lymphocytes and hepatocytes. This study aimed to evaluate the effect of LIF on natural killer group 2 member D (NKG2D) receptors expression and presentation on natural killer (NK) cells. For this purpose, peripheral blood mononuclear cells taken from 4 young male healthy blood donors were isolated and the effect of LIF (25 ng/mL) after 12, 24, and 48 hours of incubation, on NKG2D receptors expression and presentation was investigated using flow cytometry and real-time polymerase chain reaction (PCR). All of the steps of the experiment were performed in duplicate. After periods of 12, 24, and 48 hours, LIF reduced both the expression and presentation of the NKG2D receptor on NK cells. The results suggest that this cytokine has a direct modulating activity on the body’s immune response through suppression of NKG2D receptor expression and presentation on NK cells.
Keywords :
Flow cytometry , Leukemia inhibitory factor , Natural killer cells , NK cell lektin , like reseptor subfamily K
Journal title :
Iranian Journal of Allergy, Asthma and Immunology
Journal title :
Iranian Journal of Allergy, Asthma and Immunology
