Title of article :
Comprehensive Analysis of Differently Expressed and Methylated Genes in Preeclampsia
Author/Authors :
Xu, Wenyi Department of Obstetrics and Gynecology - Tongren Hospital - Shanghai Jiao Tong University School of Medicine - Shanghai, China , Ru, Ping Department of Obstetrics and Gynecology - Tongren Hospital - Shanghai Jiao Tong University School of Medicine - Shanghai, China , Gu, Zhuorong Department of Obstetrics and Gynecology - Tongren Hospital - Shanghai Jiao Tong University School of Medicine - Shanghai, China , Zhang, Ruoxi Department of Obstetrics and Gynecology - Tongren Hospital - Shanghai Jiao Tong University School of Medicine - Shanghai, China , Pang, Xixia Department of Obstetrics and Gynecology - Kongjiang Hospital - Shanghai, China , Huang, Yi Department of Life Science - Sichuan Agricultural University - Sichuan, China , Liu, Zhou Department of Health Sciences Affiliated Zhoupu Hospital - Shanghai University of Medicine - Shanghai, China , Liu, Ming Department of Obstetrics and Gynecology - Tongren Hospital - Shanghai Jiao Tong University School of Medicine - Shanghai, China
Abstract :
Preeclampsia (PE) is one of the mainly caused maternal and infant incidences and mortalities worldwide. However, the
mechanisms underlying PE remained largely unclear. The present study identified 1716 high expressions of gene and 2705 low
expressions of gene using GSE60438 database, and identified 7087 hypermethylated and 15120 hypomethylated genes in
preeclampsia using GSE100197. Finally, 536 upregulated genes with hypomethylation and 322 downregulated genes with
hypermethylation were for the first time revealed in PE. Gene Ontology (GO) analysis revealed that these genes were associated
with peptidyl-tyrosine phosphorylation, skeletal system development, leukocyte migration, transcription regulation, T cell
receptor and IFN-γ-involved pathways, innate immune response, signal transduction, cell adhesion, angiogenesis, and
hemopoiesis. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis demonstrated that aberrantly methylated
differentially expressed genes were involved in regulating adherens junction, pluripotency of stem cell regulation, immune
processing, T cell receptor and NF-κB pathways, HTLV-I and HSV infections, leishmaniasis, and NK-induced cytotoxicity.
Protein-protein interaction (PPI) network analysis identified several hub networks and key genes, including MAPK8, CCNF,
CDC23, ABL1, NF1, UBE2E3, CD44, and PIK3R1. We hope these findings will draw more attention to these hub genes in
future PE studies.
Keywords :
Genes , Methylated , KEGG , HTLV-I
Journal title :
Computational and Mathematical Methods in Medicine
