Author/Authors :
Chen, Jianxin Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education - Fujian Provincial Key Laboratoryof Photonics Technology - Fujian Normal University - Fuzhou 350007, China , Xi, Gangqin Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education - Fujian Provincial Key Laboratoryof Photonics Technology - Fujian Normal University - Fuzhou 350007, China , Chen, Zhong Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education - Fujian Provincial Key Laboratoryof Photonics Technology - Fujian Normal University - Fuzhou 350007, China , He, Jiajia Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education - Fujian Provincial Key Laboratoryof Photonics Technology - Fujian Normal University - Fuzhou 350007, China , Ren, Wenjiao Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education - Fujian Provincial Key Laboratoryof Photonics Technology - Fujian Normal University - Fuzhou 350007, China , Shen, Tingfeng Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education - Fujian Provincial Key Laboratoryof Photonics Technology - Fujian Normal University - Fuzhou 350007, China , Cao, Ning Department of Plastic Surgery - Zhangzhou Affiliated Hospital of Fujian Medical University- Zhangzhou - 363000 Fujian, China , Guo, Wenhui Department of Breast Surgery - Fujian Medical University union Hospital - Fuzhou 350001, China , Wang, Chuan Department of Breast Surgery - Fujian Medical University union Hospital - Fuzhou 350001, China , Kang, Deyong Department of Pathology - Fujian Medical University union Hospital - Fuzhou 350001, China
Abstract :
Bloodvesselsaretheimportantcomponentsofthecirculatorysystemsthattransportbloodthroughoutthehumanbodyandmaintainthehomeostasisofphysiologicaltissues .Pathologically,bloodvesselsareoftenaffectedbydiseases,leadingtotheformationofunstable,irregular,andhyperpermeablebloodvessels.Inthetumormicroenvironment,abnormalleakageoftumorbloodvesselsisrelatedtothehistologicalgradeandmalignantpotentialoftumorsandmayalsofacilitatemetastasisofcancer.Visualdiagnosisofbloodvesselsisveryimportantforustounderstandtheoccurrenceanddevelopmentofdiseases. Multiphotonmicroscopy(MPM) isapotentiallabel-freediagnostictoolbasedonsecondharmonicgeneration (SHG) andtwo-photonexcitedfluorescence(TPEF).MPMcaneffectivelyobservethemorphologicalchangesofbiologicaltissuesatthemolecularandcellularlevels.Inthiswork,wedemonstratethatlabel-free MPM canbeusedtovisualizethemicrostructureofbloodvesselsinhumannormalbreastandbreasttumortissue. Moreover,MPM canmonitorthechangesofbloodvesselsintumormicroenvironment.TheseresultsshowthattheMPMwillbecomeapromisingtechniqueforclinicianstostudythepropertiesofthemicrostructureofthebloodvessels
Keywords :
Label-Free Imaging , Blood Vessels , Human Normal Breast , Breast Tumor Tissue Using Multiphoton Microscopy , TPEE , SHG
