Title of article
Resolution-Free Accurate DNA Contour Length Estimation fromAtomic Force Microscopy Images
Author/Authors
Hsaio, Ming-Chi Mechanical Engineering - National Taiwan University of Science and Technology, Taiwan , Chang, Peter I. Mechanical Engineering - National Taiwan University of Science and Technology, Taiwan
Pages
15
From page
1
To page
15
Abstract
Thisresearchpresentedanaccurateandefficientcontourlengthestimationmethoddevelopedfor DNA digitalcurvesacquiredfromAtomicForceMicroscopy(AFM)images. ThisautomationmethodiscalibratedagainstdifferentAFMresolutionsandidealtobeextendedtoalldifferentkindsofbiopolymersamples,encompassingalldifferentsamplestiffnesses. Themethodologyconsidersthedigitalcurvelocalgeometricrelationship,asthesedigitalshapesegmentsandpixelconnectionsrepresenttheactualmorphologyofthebiopolymersampleasitisbeingimagedfromtheAFMscanning. Inordertoincorporatethetruelocalgeometryrelationshipthatisembeddedinthecontinuousformoftheoriginalsample,oneneedstofindthisgeometrycounterpartinthedigitizedimage. Thiscounterpartisrealizedbytakingtheskeletonbackboneofthesamplecontourandbyusingthesedigitizedpixels’connectionrelationshiptofinditslocalshaperepresentation.Inthisresearch,oneusesthe8-connectFreemanChainCode(CC) todescribethedirectionalconnectionbetween DNA imagepixels,inordertoaccountforthelocalshapesoffourconnectedpixels.Theresultisanovelshapenumber(SN)systemderivedfromCC,whichisafullyautomatedalgorithmthatcanbeappliedtoDNAsamplesofanylengthforaccurateestimation,withefficientcomputationalcost. Thisshape-wiseconsiderationisweightedtomodifythelocallengthwithgreatprecision,accountingforallthedifferentmorphologiesofthebiopolymersample, andresultedwithaccuratelengthestimation,astheerrorfallsbelow0.07%, anorderofmagnitudeimprovementcomparedtopreviousfindings.
Keywords
Resolution-Free Accurate DNA Contour Length Estimation , Atomic Force Microscopy Images , TheAtomicForceMicroscopy(AFM)
Journal title
Scanning
Serial Year
2019
Record number
2613418
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