Title of article :
Conformational Distortions of the Red Blood Cell Spectrin Matrix Nanostructure in Response to Temperature Changes In Vitro
Author/Authors :
Kozlova, Elena V.A. Negovsky Research Institute of General Reanimatology - Federal Research and Clinical Center of Intensive Care Medicineand Rehabilitology - Moscow, Russia , Chernysh, Aleksandr V.A. Negovsky Research Institute of General Reanimatology - Federal Research and Clinical Center of Intensive Care Medicineand Rehabilitology - Moscow, Russia , Sergunova, Viktoria V.A. Negovsky Research Institute of General Reanimatology - Federal Research and Clinical Center of Intensive Care Medicineand Rehabilitology - Moscow, Russia , Manchenko, Ekaterina V.A. Negovsky Research Institute of General Reanimatology - Federal Research and Clinical Center of Intensive Care Medicineand Rehabilitology - Moscow, Russia , Moroz, Viktor V.A. Negovsky Research Institute of General Reanimatology - Federal Research and Clinical Center of Intensive Care Medicineand Rehabilitology - Moscow, Russia , Kozlov , Aleksandr Sechenov First Moscow State Medical University (Sechenov University) - Moscow, Russia
Abstract :
Thespectrinmatrixisastructuralelementofredbloodcells (RBCs). Assuch,itaffectsRBCmorphology, membranedeformability,nanostructure, stiffness,and,ultimately,therheologicalpropertiesofblood. However,littleisknownabouthowtemperatureaffectsthespectrinmatrix.Inthisstudy,thenanostructureofthespectrinnetworkwasrecordedbyatomicforcemicroscopy. WedescribehowthenanostructureoftheRBCspectrinmatrixchangesfromaregularnetworktoachaoticpatternfollowinganincreaseintemperaturefrom20to50°C.At20–37°С, thespectrinnetworkformedaregularstructurewithdimensionsoftypically150±60nm. At42–43°С,83%of thespectrinnetworkassumedanirregularstructure.Finally,at49–50°Сthechaoticpatternwasobserved,andnoquantitativeestimatesofthespectrinstructure’sparameterscouldbemade. Theseresultscanbeusefulforbiophysicalstudiesonthedestructionofthespectrinnetworkunderpathologicalconditions,aswellasforinvestigatingcellmorphologyandbloodrheologyindifferentdiseases
Keywords :
Conformational Distortions , Red Blood Cell Spectrin Matrix Nanostructure , Temperature Changes In Vitro , Redbloodcell(RBC)
