Title of article :
Analgesic, Anti-Inflammatory, and Antioxidant Activities of Byrsonima duckeana W. R. Anderson (Malpighiaceae)
Author/Authors :
dos Santos Verdam, Maria Christina Department of Pharmacy - Federal University of Parana - Curitiba - PR - Brazil ´ - Faculty of Pharmacy - Federal University of Rio de Janeiro - Rio de Janeiro - RJ - Brazil , Guilhon-Simplicio, Fernanda Faculty of Pharmaceutical Sciences - Federal University of Amazonas - Manaus - AM - Brazil , de Andrade, Kleyton Cardoso Faculty of Pharmaceutical Sciences - Federal University of Amazonas - Manaus - AM - Brazil , Meira Fernandes, Karina Lorena Center of Biological and Health Sciences - Pontifical Catholic University of Parana - Curitiba - PR - Brazil , Marques Machado, Tallita Faculty of Pharmaceutical Sciences - Federal University of Amazonas - Manaus - AM - Brazil , Araújo da Silva, Felipe Moura Department of Chemistry - Federal University of Amazonas - Manaus - AM - Brazil , de Souza, Mayane Pereira Department of Chemistry - Federal University of Amazonas - Manaus - AM - Brazil , Ferreira Koolen, Hector Henrique De Mp Ster Mass Spectrometry Group - Amazonas State University - Manaus - AM - Brazil , da Silva Paula, Cristiane Department of Pharmacy - Federal University of Parana - Curitiba - PR - Brazil , Konopatzki Hirota, Beatriz Cristina Department of Pharmacy - Federal University of Parana - Curitiba - PR - Brazil , de Oliveira, Vinícius Bednarczuk Department of Pharmacy - Federal University of Parana - Curitiba - PR - Brazil , Sasaki Miyazaki, Cristina Mayumi Department of Pharmacy - Federal University of Parana - Curitiba - PR - Brazil , Kalegari, Milena Department of Pharmacy - Federal University of Parana - Curitiba - PR - Brazil , Dallarmi Miguel, Marilis Department of Pharmacy - Federal University of Parana - Curitiba - PR - Brazil , Stuelp-Campelo, Patricia Maria Center of Biological and Health Sciences - Pontifical Catholic University of Parana - Curitiba - PR - Brazil , Gomes Miguel, Obdulio Department of Pharmacy - Federal University of Parana - Curitiba - PR - Brazil
Abstract :
Background. Byrsonima is a promising neotropical genus, rich in flavonoids and triterpenes, with several proven pharmacological properties. Nevertheless, Byrsonima duckeana W. R. Anderson is an Amazonian species almost not studied. Objective. To assess the antioxidant, anti-inflammatory, and analgesic activities of Byrsonima duckeana leaves. Materials and Methods. We analyzed an
ethanol extract and its fractions for polyphenol content and UHPLC-MS/MS, phosphomolybdenum, DPPH, TBARS antioxidant
tests, formalin-induced pain, carrageenan-induced peritonitis, acetic acid-induced abdominal writhings, and hot plate assays.
Results. All the samples showed high polyphenol content and antioxidant capacity in the phosphomolybdenum, DPPH, and TBARS
tests. We identified ethyl gallate, quinic acid, gallic acid, catechin, epicatechin, quercetrin, and quercetin in the samples. B. duckeana
was able to reduce leukocyte migration in the carrageenan-induced peritonitis by 43% and the licking time in the formalin test by
57%. In the acetic acid-induced writhing test, the chloroform (FCL) and ethyl acetate (FEA) fractions were the most active samples.
FEA was selected for the hot plate test, where all the dosages tested (5, 50, and 200 mg⋅kg−1) showed significant analgesic activity.
Conclusion. B. duckeana has interesting analgesic and antioxidant activities, due to its high phenolic content, especially phenolic acids.
Keywords :
Analgesic , Anti-Inflammatory , Antioxidant Activities , Byrsonima duckeana W. R. Anderson , Malpighiaceae
Journal title :
The Scientific World Journal
