Title of article :
Nonlinear Biomechanical Characteristics of Deep Deformation ofNative RBC Membranes in Normal State and underModifier Action
Author/Authors :
Moroz, Viktor FederalResearchandClinicalCenterofIntensiveCareMedicineandRehabilitology - V.A.NegovskyResearchInstituteofGeneralReanimatology - Moscow, Russia , Kozlova, Elena FederalResearchandClinicalCenterofIntensiveCareMedicineandRehabilitology - V.A.NegovskyResearchInstituteofGeneralReanimatology - Moscow, Russia , Chernysh, Aleksandr FederalResearchandClinicalCenterofIntensiveCareMedicineandRehabilitology - V.A.NegovskyResearchInstituteofGeneralReanimatology - Moscow, Russia , Manchenko, Ekaterina FederalResearchandClinicalCenterofIntensiveCareMedicineandRehabilitology - V.A.NegovskyResearchInstituteofGeneralReanimatology - Moscow, Russia , Sergunova, Viktoria FederalResearchandClinicalCenterofIntensiveCareMedicineandRehabilitology - V.A.NegovskyResearchInstituteofGeneralReanimatology - Moscow, Russia
Abstract :
Theabilityofmembranesofnativehumanredbloodcells(RBCs)tobendintothecelltoadepthcomparableinsizewithphysiologicaldeformationswasevaluated.Forthis,themethodsofatomicforcemicroscopyandatomicforcespectroscopywereused.Nonlinearpatternsofdeepdeformation(upto600nm)ofRBCmembraneswerestudiedinnormalstateandundertheactionofmodifiers:fixator(glutaraldehyde),naturaloxidant(hemin),andexogenousintoxicator(zincions),invitro.TheexperimentaldependencesofmembranebendingforcontrolRBC(normal)wereapproximatedbytheHertzmodeltoadepthupto600nm.TheglutaraldehydefixatorandmodifiersincreasedtheabsolutevalueofYoung’smodulusofmembranesandchangedtheexperimentaldependencesofprobeindentationintothecells.UptosomedepthhHz,theforcecurveswereapproximatedbytheHertzmodel,andfordeeperindentationsh>hHz,thedegreeofthepolynomialfunctionwaschanged,themembranestiffnessincreased,andthepatternofindentationbecameanotheranddidnotobeytheHertzmodel.QuantitativecharacteristicsofnonlinearexperimentaldependenceswerecalculatedfordeepbendingofRBCmembranesbyapproximatingthembythedegreepolynomialfunction
Keywords :
Nonlinear Biomechanical Characteristics , Deep Deformation , Native RBC Membranes , Normal State , Modifier Action , RBCs
