Title of article :
AFM and FluidFM Technologies: Recent Applications in Molecularand Cellular Biology
Author/Authors :
Mazouzi, Driss Materials - NaturalSubstances - EnvironmentandModelingLaboratory - MultidisciplinaryFacultyofTaza - UniversitySidiMohammedBenAbdellah - Fez, Morocco , Amarouch, Mohamed Yassine Materials - NaturalSubstances - EnvironmentandModelingLaboratory - MultidisciplinaryFacultyofTaza - UniversitySidiMohammedBenAbdellah - Fez, Morocco , Hilaly, Jaouad El Materials - NaturalSubstances - EnvironmentandModelingLaboratory - MultidisciplinaryFacultyofTaza - UniversitySidiMohammedBenAbdellah - Fez, Morocco
Abstract :
Atomicforcemicroscopy(AFM)isawidelyusedimagingtechniqueinmaterialsciences.Afterbecomingastandardsurface-imagingtool,AFMhasbeenproventobeusefulinaddressingseveralbiologicalissuessuchasthecharacterizationofcellorganelles,quantificationofDNA-proteininteractions,celladhesionforces,andelectromechanicalpropertiesoflivingcells.AFMtechniquehasundergonemanysuccessfulimprovementssinceitsinvention,includingfluidicforcemicroscopy(FluidFM),whichcombinesconventionalAFMwithmicrochanneledcantileversforlocalliquiddispensing.Thistechnologypermittedtoovercomechallengeslinkedtosingle-cellanalyses.Indeed,FluidFMallowsisolationandinjectionofsinglecells,force-controlledpatchclampingofbeatingcardiaccells,serialweightingofmicro-objects,andsingle-cellextractionformolecularanalyses.ThisworkaimstoreviewtherecentstudiesofAFMimplementationinmolecularandcellularbiology
Keywords :
AFM , FluidFM Technologies , Recent Applications , Molecularand Cellular Biology , Atomicforcemicroscopy(AFM)
