Identifying Two Common Types of Breast Benign Diseases Based on Multiphoton Microscopy

Multiphoton microscopy has attracted increasing attention and investigations in the field of breast cancer, based on two-photonexcited fluorescence (TPEF) and second-harmonic generation (SHG). However, the incidence of breast benign diseases is about 5to 10 times higher than breast cancer; up to 30% of women suffer from breast benign diseases and require treatment at some time intheir lives. Thus, in this study, MPM was applied to image fibroadenoma and fibrocystic lesion, which are two of the most commonbreast benign diseases. The results show that MPM has the capability to identify the microstructure of lobule and stroma in normalbreast tissue, the interaction of compressed ducts with surrounding collagen fiber in fibroadenoma, and the architecture of cystsfilled with cystic fluid in fibrocystic disease. These findings indicate that, with integration of MPM into currently accepted clinicalimaging system, it has the potential to make a real-time diagnosis of breast benign diseases in vivo, as well as breast cancer.