Author/Authors :
Addai-Mensah, Otchere Department of Medical Laboratory Technology - Faculty of Allied Health Sciences - Kwame Nkrumah University of Science and Technology - Kumasi - Ghana , Gyamfi, Danie Department of Medical Laboratory Technology - Faculty of Allied Health Sciences - Kwame Nkrumah University of Science and Technology - Kumasi - Ghana , Agyei Amponsah, Francis St. John of God Hospital - Duayaw Nkwanta - Sunyani - Ghana , Annani-Akollor, Max Efui Department of Molecular Medicine - School of Medical Sciences - Kwame Nkrumah University of Science and Technology - Kumasi - Ghana , Owusu Danquah, Kwabena Department of Medical Laboratory Technology - Faculty of Allied Health Sciences - Kwame Nkrumah University of Science and Technology - Kumasi - Ghana , Boateng, Lillian Department of Medical Laboratory Technology - Faculty of Allied Health Sciences - Kwame Nkrumah University of Science and Technology - Kumasi - Ghana , Owiredu, Eddie-Williams Department of Medical Laboratory Technology - Faculty of Allied Health Sciences - Kwame Nkrumah University of Science and Technology - Kumasi - Ghana , Afriyie, Edward Y Department of Haematology - Komfo Anokye Teaching Hospital - Kumasi - Ghana , Vikpebah Duneeh, Richard Department of Haematology - Komfo Anokye Teaching Hospital - Kumasi - Ghana , Asare, Renate Department of Haematology - Komfo Anokye Teaching Hospital - Kumasi - Ghana , Ofosu Ntiamoah, David Department of Haematology - Komfo Anokye Teaching Hospital - Kumasi - Ghana , Boateng, Richard Department of Serology - Komfo Anokye Teaching Hospital - Kumasi - Ghana
Abstract :
Introduction
The pathophysiology of malaria-related anaemia is not fully understood although increased destruction of parasitized and nonparasitized erythrocytes, as well as inadequate erythropoiesis, has been proposed. Circulating antierythropoietin (anti-EPO) antibodies have also been implicated in malaria and malaria-related anaemia in mice. However, studies on this association have not been investigated in humans. This study therefore determined the prevalence of anti-EPO antibody production and assessed its association with malaria and malaria-related anaemia in humans.
Methods
A total of 86 children aged 1-10 years (57 children with malaria serving as the case group and 29 healthy children serving as control), all residents of Duayaw Nkwanta, Ghana, were recruited for this case-control study. Venous blood was collected for thick and thin films for malaria microscopy, full blood count by automated haematology analyzer, and antierythropoietin antibody and erythropoietin estimation by sandwich ELISA method.
Results
Out of the 86 participants recruited, only 3 (3.5%) were positive for anti-EPO antibody; 2.3% of the case group; and 1.2% of the control group. There was no association between the cases and the controls in the production of anti-EPO antibodies. Erythropoietin concentration was significantly higher in malaria-related anaemic subjects (p=0.032).
Conclusion
Antierythropoietin antibodies are not associated with malaria infection and malaria-related anaemia in humans. Erythropoietin concentration is associated with malaria-related anaemia.
Keywords :
Antierythropoietin Antibody Production , Malaria , Anaemia , Humans
