Author/Authors :
Chomiski, Verônica Department of Surgery - Universidade Federal de São Paulo (UNIFESP), Sao Paulo-SP, Brazil , Gragnani, Alfredo Department of Surgery - Universidade Federal de São Paulo (UNIFESP), Sao Paulo-SP, Brazil , Bonucci, Jéssica Department of Surgery - Universidade Federal de São Paulo (UNIFESP), Sao Paulo-SP, Brazil , Correa, Silvana Aparecida Alves Department of Surgery - Universidade Federal de São Paulo (UNIFESP), Sao Paulo-SP, Brazil , Noronha, Samuel Marcos Ribeiro de Department of Surgery - Universidade Federal de São Paulo (UNIFESP), Sao Paulo-SP, Brazil , Ferreira, Lydia Masako UNIFESP, Sao Paulo-SP, Brazil
Abstract :
PURPOSE:
To evaluate the effect of keratinocyte growth factor (KGF) treatment on the expression of wound-healing-related genes in cultured keratinocytes from burn patients.
METHODS:
Keratinocytes were cultured and divided into 4 groups (n=4 in each group): TKB (KGF-treated keratinocytes from burn patients), UKB (untreated keratinocytes from burn patients), TKC (KGF-treated keratinocytes from controls), and UKC (untreated keratinocytes from controls). Gene expression analysis using quantitative polymerase chain reaction (qPCR) array was performed to compare (1) TKC versus UKC, (2) UKB versus UKC, (3) TKB versus UKC, (4) TKB versus UKB, (5) TKB versus TKC, and (6) UKB versus TKC.
RESULTS:
Comparison 1 showed one down-regulated and one up-regulated gene; comparisons 2 and 3 resulted in the same five down-regulated genes; comparison 4 had no significant difference in relative gene expression; comparison 5 showed 26 down-regulated and 7 up-regulated genes; and comparison 6 showed 25 down-regulated and 11 up-regulated genes.
CONCLUSION:
There was no differential expression of wound-healing-related genes in cultured primary keratinocytes from burn patients treated with keratinocyte growth factor.
Keywords :
Burns , Gene Expression , Wound Healing , Keratinocytes
