Title of article :
Chemical Composition and Mechanism of Vibriocidal Action of Essential Oil from Resin of Protium heptaphyllum
Author/Authors :
Lima Mendes, Josiane UNINTA College (UNINTA) - Sobral - Ceara´ - Brazil , de Arau´jo, Thiago Ferreira State University of the Tocantina Region of Maranhão - Imperatriz - Maranhão - Brazil , de Carvalho, Ma´rio Geraldo Chemistry Department - Federal Rural University of Rio de Janeiro - Serop´edica - Rio de Janeiro - Brazil , Eduardo Aragão Catunda Ju´nior, Francisco State University of the Tocantina Region of Maranhão - Imperatriz - Maranhão - Brazil , Albuquerque Costa, Renata UNINTA College (UNINTA) - Sobral - Ceara´ - Brazil
Abstract :
Protium heptaphyllum is a plant widely distributed in Brazilian ecosystems that produce a resin which has pharmacological activities. In this study, the chemical composition, antimicrobial and antibiofilm activity, and the possible mechanism of action against the bacterium V. parahaemolyticus of essential oil from P. heptaphyllum (EOPH) were investigated. Twenty-two components were detected in EOPH, and β-phellandrene (60.68%) had the majority. The inhibition halo, MIC, and MBC values were 11 mm, 2 mg/mL, and 8 mg/mL, respectively. Biofilm biomass inhibition and biomass reduction of the preformed biofilm were detected at 4 mg/mL EOPH concentration. The assays of cell constituent release and membrane permeability indicated that EOPH may disrupt the cell membrane, leading to leakage of intracellular constituent as reducing sugars and materials with an absorbance of 260 nm.
Keywords :
Chemical Composition , Mechanism , Vibriocidal Action , Essential Oil , Protium heptaphyllum
Journal title :
The Scientific World Journal
