Author/Authors :
Carvalho, Camila Albuquerque Melo de Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - Universidade de São Paulo (USP) - Universidade Federal de Alagoas (UFAL)Ribeirao PretoSP, Brazil , Tirapelli, Daniela Pretti da Cunha Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - USP, Brazil , Rodrigues, Andressa Romualdo Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - USP, Brazil , Lizarte, Fermino Sanches Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - USP, Brazil , Novais, Paulo Cézar Department of Health Sciences - Universidade de Marília (UNIMAR), Brazil , Silva, Jairo Pinheiro Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - USP, Brazil , Carlotti, Carlos Gilberto Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - USP, Brazil. , Colli, Benedicto Oscar Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - USP, Brazil. , Tirapelli, Luís Fernando Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - USP, Brazil.
Abstract :
PURPOSE:
To evaluated histopathological changes, morphometric and expression of proteins CASPASE-3, BCL-2 and XIAP related to apoptosis in the cerebellum after induction of temporary focal cerebral ischemia followed by reperfusion, with or without a model of chronic alcoholism.
METHODS:
Fifty Wistar rats were used and divided into: control group (C), sham group (S), ischemic group (I), alcoholic group (A), and ischemic and alcoholic group (IA). The cerebellum samples collected were stained for histopathological and morphometric analysis and immunohistochemistry study.
RESULTS:
Histopathological changes were observed a greater degree in animals in groups A and IA. The morphometric study showed no difference in the amount of cells in the granular layer of the cerebellum between the groups. The expression of CASPASE-3 was higher than BCL-2 and XIAP in the groups A and IA.
CONCLUSION:
We observed correlation between histopathological changes and the occurrence of apoptosis in cerebellar cortex.
Keywords :
Ischemia , Alcoholism , Cerebellum , Apoptosis , Morphology , Rats
