Author/Authors :
Shanthini, Thamaraiselvan Antibiotic Resistance and Phage Therapy Laboratory, Department of Biomedical Sciences, School of Bioscience and Technology, Vellore Institute of Technology (VIT), Vellore, Tamil Nadu, India , Manohar, Prasanth Antibiotic Resistance and Phage Therapy Laboratory, Department of Biomedical Sciences, School of Bioscience and Technology, Vellore Institute of Technology (VIT), Vellore, Tamil Nadu, India , Samna, Sagadevan Antibiotic Resistance and Phage Therapy Laboratory, Department of Biomedical Sciences, School of Bioscience and Technology, Vellore Institute of Technology (VIT), Vellore, Tamil Nadu, India , Srividya, Rajendran Antibiotic Resistance and Phage Therapy Laboratory, Department of Biomedical Sciences, School of Bioscience and Technology, Vellore Institute of Technology (VIT), Vellore, Tamil Nadu, India , Bozdogan, Bulent Department of Microbiology - Recombinant DNA and Protein Research Center (REDPROM) - Adnan Menderes University, Turkey , Rameshpathy, Manian Department of Biotechnology - School of Biosciences and Technology - Vellore Institute of Technology (VIT), Vellore, Tamil Nadu, India , Ramesh, Nachimuthu Antibiotic Resistance and Phage Therapy Laboratory, Department of Biomedical Sciences, School of Bioscience and Technology, Vellore Institute of Technology (VIT), Vellore, Tamil Nadu, India
Abstract :
Wastewater has become a potential habitat for multi-drug-resistant bacteria. The present study aims to screen for the presence of carbapenem-resistant bacteria in sewage water samples collected from hospital and non-hospital sources. From a total of 19 sewage water samples collected, 100 carbapenem-resistant non-lactose-fermenting Gram-negative bacteria (CR-NF-GNB) were isolated using MacConkey agar cultured with 8 mg l−1 of meropenem. On screening for beta-lactamase resistance genes (bla NDM, bla OXA-48-like, bla IMP, bla VIM and bla KPC), one isolate, Ochrobactrum intermedium , was found to carry the plasmid-borne bla OXA-48-like gene. To the best of our knowledge, we provide the first report of the rare and emerging opportunistic pathogen Ochrobactrum intermedium encoding the OXA-181 gene in its plasmid.
Keywords :
Ochrobactrum intermedium , carbapenem-resistant , multi-drug resistance , carbapenemase , OXA , Gram-negative bacteria
