Author/Authors :
Zelepukin, I.V Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry - Russian Academy of Sciences, Russia , Shipunova, V.O Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry - Russian Academy of Sciences, Russia , Mirkasymov, A.B Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry - Russian Academy of Sciences, Russia , Nikitin, P.I Prokhorov General Physics Institute - Russian Academy of Sciences, Russia , Nikitin, M.P Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry - Russian Academy of Sciences, Russia , Deyev, S.M Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry - Russian Academy of Sciences, Russia
Abstract :
The creation of markers that provide both visual and quantitative information is of considerable im-portance for the mapping of tissue macrophages and other cells. We synthesized magnetic and magneto-fluores-cent nanomarkers for the labeling of cells which can be detected with high sensitivity by the magnetic particle quantification (MPQ) technique. For stabilization under physiological conditions, the markers were coated with a dense silica shell. In this case, the size and zeta-potential of nanoparticles were controlled by a modified Stober reaction. Also, we developed a novel facile two-step synthesis of carboxylicacid-functionalized magnetic SiO2 nanoparticles, with a carboxyl polymer shell forming on the nanoparticles before the initiation of the Stober reaction. We extensively character-ized the nanomarkers by transmission electron microscopy, electron microdiffraction, and dynamic and electrophoretic light scattering. We also studied the nanoparticle cellular uptake by various eukaryotic cell lines.
Keywords :
cell labeling , silicium dioxide , magnetic detection , surface modification , magnetic nanoparticles
