Author/Authors :
Kaneko, Mei Department of Developmental Medical Sciences - Graduate School of Medicine - The University of Tokyo, Bunkyo, Tokyo, Japan , Takanashi, Sayaka Department of Developmental Medical Sciences - Graduate School of Medicine - The University of Tokyo, Bunkyo, Tokyo, Japan , Inoue, Mana Department of Developmental Medical Sciences - Graduate School of Medicine - The University of Tokyo, Bunkyo, Tokyo, Japan , Sakiyama, Hiroshi Sakiyama Pediatric Clinic, Fuchu, Tokyo, Japan , Okitsu, Shoko Division of Microbiology - Department of Pathology and Microbiology, School of Medicine, Nihon University, Itabashi, Tokyo, Japan , Mizuguchi, Masashi Department of Developmental Medical Sciences - Graduate School of Medicine - The University of Tokyo, Bunkyo, Tokyo, Japan , Ushijima, Hiroshi Division of Microbiology - Department of Pathology and Microbiology, School of Medicine, Nihon University, Itabashi, Tokyo, Japan
Abstract :
Strains of Rotarix, a live attenuated monovalent oral rotavirus vaccine, replicate in the intestine and are shed for about one month in immunocompetent recipients. The current study aimed to identify genetic changes of shed strains to reveal any significant mutations and their clinical impact on recipients. Stool samples of recipients of the first dose of Rotarix were sequentially collected for one month from the day of administration. Sequence analyses of the VP7 gene in eight recipients revealed five amino acid substitutions. Among them, two were observed in aa123, which is located in antigenic region 7-1a. Since there were no associated clinical symptoms, the genetic changes were unlikely to have caused reversion of pathogenicity of vaccine strain. Of interest, the virus in one case became closer to wild-type rotavirus via an amino acid change at aa123 occurring 14 days after administration, which might have resulted from multiple replications and long-term shedding of the vaccine strain.
Keywords :
rotavirus , vaccine , shedding , VP7 gene , mutation
