Author/Authors :
Zolotarev, N.A. Institute of Gene Biology - Russian Academy of Sciences, Vavilova str. 34/5, Moscow, 119334, Russia , Maksimenko, O.G. Institute of Gene Biology - Russian Academy of Sciences, Vavilova str. 34/5, Moscow, 119334, Russia , Georgiev, P.G. Institute of Gene Biology - Russian Academy of Sciences, Vavilova str. 34/5, Moscow, 119334, Russia , Bonchuk, A.N. Institute of Gene Biology - Russian Academy of Sciences, Vavilova str. 34/5, Moscow, 119334, Russia
Abstract :
Many arthropod zinc-finger transcription factors contain a N-terminal domain called ZAD (Zinc-fin-ger Associated Domain), which consists of four cysteines coordinating a single zinc ion. Dimerization ability has been shown for several ZAD-domains. The functional role of this domain is poorly understood. In this paper, we demonstrate that a point mutation within the ZAD-domain of the Zw5 insulator protein disrupts its nuclear lo-calization without affecting its dimerization ability. The importance of the ZAD-domain for nuclear localization has also been shown for the Pita and Grauzone proteins. Therefore, one of the ZAD-domain functions is control of the nuclear localization of transcription factors.
