Author/Authors :
Aliev, T.K Lomonosov Moscow State University - Department of Chemistry, Moscow, Russia , Dement’yeva, I.G Russian Research Center for Molecular Diagnostics and Therapy, Moscow, Russia , Toporova, V.A Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry - Russian Academy of Sciences, Moscow, Russia , Argentova, V.V Lomonosov Moscow State University - Faculty of Biology,Moscow, Russia , Pozdnyakova, L.P Russian Research Center for Molecular Diagnostics and Therapy, Moscow, Russia , Bokov, M.N Russian Research Center for Molecular Diagnostics and Therapy, Moscow, Russia , Votchitseva, Yu.A Lomonosov Moscow State University - Faculty of Biology, Moscow, Russia , Dolgikh, D.A Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry - Russian Academy of Sciences, Moscow, Russia , Varfolomeyev, S.D Lomonosov Moscow State University - Department of Chemistry, Moscow, Russia , Sveshnikov, P.G Russian Research Center for Molecular Diagnostics and Therapy, Moscow, Russia , Kirpichnikov, M.P Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry - Russian Academy of Sciences, Moscow, Russia
Abstract :
We obtained recombinant variants of human antibody FI6 broadly specific to hemagglutinins of the influenza A virus. On the basis of a bi-promoter (CMV, hEF1-HTLV) vector, we developed genetic constructs for the expression of the heavy and light chains of the immunoglobulins of IgA1-, IgA2m1-, and IgG-isotypes. Following transfection and selection, stable Chinese hamster ovary (CHO) cell lines were produced. The antibod-ies of IgA1-, IgA2m1-, and IgG-isotypes were purified from culture media. We performed an immunochemical characterization and studied their interactions with influenza A strains of the H1N1- and H3N2-subtypes. It was shown that recombinant FI6 variants of the IgA-isotype retain the properties of the parental IgG antibody to demonstrate specificity to all the strains tested. The strongest binding was observed for the H1N1 subtype, which belongs to hemagglutinins of phylogenetic group I.
Keywords :
recombinant antibodies , IgA2m1 , IgA1 , immunoglobulin A , broadly neutralizing antibodies , Influenza A virus
