Author/Authors :
Virtanen, Helena University of Turku - Turku, Finland , Silvola, Johanna M. U University of Turku - Turku, Finland , Autio, Anu University of Turku - Turku, Finland , Li, Xiang-Guo Abo Akademi University - Turku, Finland , Liljenbäck, Heidi University of Turku - Turku, Finland , Hellberg, Sanna University of Turku - Turku, Finland , Siitonen, Riikka University of Turku - Turku, Finland , Ståhle, Mia University of Turku - Turku, Finland , Käkelä, Meeri University of Turku - Turku, Finland , Airaksinen, Anu J Department of Chemistry - University of Helsinki - Helsinki, Finland , Helariutta, Kerttuli Department of Chemistry - University of Helsinki - Helsinki, Finland , Tolvanen, Tuula Turku University Hospital - Turku, Finland , Veres, Tibor Z. University of Turku - Turku, Finland , Saraste, Antti University of Turku - Turku, Finland , Knuuti, Juhani University of Turku - Turku, Finland , Jalkanen, Sirpa University of Turku - Turku, Finland , Roivainen, Anne University of Turku - Turku, Finland
Abstract :
Sialic acid-binding immunoglobulin-like lectin 9 (Siglec-9) is a ligand of inflammation-inducible vascular adhesion protein-1 (VAP1).We compared 68Ga-DOTA- and 18F-fluorodeoxyribose- (FDR-) labeled Siglec-9 motif peptides for PET imaging of inflammation.
Methods. Firstly, we examined 68Ga-DOTA-Siglec-9 and 18F-FDR-Siglec-9 in rats with skin/muscle inflammation. We then studied 18F-FDR-Siglec-9 for the detection of inflamed atherosclerotic plaques in mice and compared it with previous 68Ga-DOTA-Siglec-9
results. Lastly, we estimated human radiation dosimetry from the rat data. Results. In rats, 68Ga-DOTA-Siglec-9 (SUV, 0.88 ± 0.087)
and 18F-FDR-Siglec-9 (SUV, 0.77 ± 0.22) showed comparable (𝑃 = 0.29) imaging of inflammation. In atherosclerotic mice, 18FFDR-Siglec-9 detected inflamed plaques with a target-to-background ratio (1.6 ± 0.078) similar to previously tested 68Ga-DOTASiglec-9 (𝑃 = 0.35). Human effective dose estimates for 68Ga-DOTA-Siglec-9 and 18F-FDR-Siglec-9 were 0.024 and 0.022 mSv/MBq,
respectively. Conclusion. Both tracers are suitable for PET imaging of inflammation. The easier production and lower cost of 68GaDOTA-Siglec-9 present advantages over 18F-FDR-Siglec-9, indicating it as a primary choice for clinical studies.
