Author/Authors :
Lemos, Sylvio Valença de Department of Anesthesiology - Botucatu Medical School - Universidade Estadual de São Paulo (UNESP), Botucatu-SP, Brazil , Vianna, Isabela Galvão Department of Anesthesiology - Botucatu Medical School - Universidade Estadual de São Paulo (UNESP), Botucatu-SP, Brazil , Castiglia, Yara Marcondes Machado Department of Anesthesiology - Botucatu Medical School - Universidade Estadual de São Paulo (UNESP), Botucatu-SP, Brazil , Golim, Marjorie de Assis Department of Internal Medicine - Botucatu Medical School - UNESP, Botucatu-SP, Brazil , Souza, Aparecida Vitória Gonçalves de Department of Anesthesiology - Botucatu Medical School - UNESP, Botucatu-SP, Brazil , Carvalho, Lídia Raquel de Department of Biostatistics - UNESP, Botucatu-SP, Brazil , Deffune, Elenice Department of Internal Medicine - Botucatu Medical School - UNESP, Botucatu-SP, Brazil , Nascimento, Paulo do Department of Anesthesiology - Botucatu Medical School - UNESP, Botucatu-SP, Brazil , Módolo, Norma Sueli Pinheiro Department of Anesthesiology - Botucatu Medical School - UNESP, Botucatu-SP, Brazil , Vianna, Pedro Thadeu Galvão Department of Anesthesiology - Botucatu Medical School - UNESP, Botucatu-SP, Brazil
Abstract :
Purpose:
To investigate the effects of cyclosporine A on renal ischemia-reperfusion injury during transient hyperglycemia in rats.
Methods:
In a model of ischemia-reperfusion-induced renal injury and transiently induced hyperglycemia by intraperitoneal injection of glucose, 2.5 g.kg-1, Wistar rats were anesthetized with either isoflurane or propofol and received intravenous cyclosporine A, 5 mg.kg-1, five minutes before reperfusion. Comparison groups were isoflurane and propofol sham groups and isoflurane and propofol ischemia-reperfusion-induced renal injury. Renal tubular cell viability was quantitatively assessed by flow cytometry after cell culture and classified as early apoptosis, necrotic cells, and intact cells.
Results:
Early apoptosis was significantly higher in isoflurane and propofol anesthetized animals subjected to renal ischemia-reperfusion injury when compared to both cyclosporine A treated and sham groups. Necrosis percentage was significantly higher in propofol-anesthetized animals subjected to renal ischemia-reperfusion injury. The percentage of intact cells was lower in both, isoflurane and propofol anesthetized animals subjected to renal ischemia-reperfusion injury.
Conclusion:
In a model of ischemia-reperfusion-induced renal injury, cyclosporine A, 5 m.kg-1, administered five minutes before renal reperfusion in rats with acute-induced hyperglycemia under either isoflurano or propofol anesthesia, attenuated early apoptosis and preserved viability in renal tubular cells, regardless of the anesthetic used.
Keywords :
Cyclosporine , Hyperglycemia , Reperfusion Injury , Apoptosis , Kidney , Rats
