Author/Authors :
Carvalho, Thais Inácio de Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - Universidade de São Paulo (USP), Brazil , Novais, Paulo Cezar Department of Health Sciences - Universidade de Marília (UNIMAR), Brazil , Lizarte, Fermino Sanches Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - USP, Brazil , Sicchieri, Renata Danielle Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - USP, Brazil , Rosa, Marcella Suelma Torrecillas Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - USP, Brazil , Carvalho, Camila Albuquerque Mello de Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - USP, Brazil , Tirapelli, Daniela Pretti da Cunha Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - USP, Brazil , Peria, Fernanda Maris Department of Medical Clinical - School of Medicine of Ribeirão Preto - USP, Brazil , Rocha, José Joaquim Ribeiro da Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - USP, Brazil , Féres, Omar Department of Surgery and Anatomy - School of Medicine of Ribeirao Preto - USP, Brazil
Abstract :
Purpose:
To evaluate the expression of EGFR, KRAS genes, microRNAs-21 and 203 in colon and rectal cancer samples, correlated with their age at diagnosis, histological subtype, value of pretreatment CEA, TNM staging and clinical outcome.
Methods:
Expression of genes and microRNAs by real time PCR in tumor and non-tumor samples obtained from surgical treatment of 50 patients.
Results:
An increased expression of microRNAs-21 and 203 in tumor samples in relation to non-tumor samples was found. There was no statistically significant difference between the expression of these genes and microRNAs when compared to age at diagnosis and histological subtype. The EGFR gene showed higher expression in relation to the value of CEA diagnosis. The expression of microRNA-203 was progressively lower in relation to the TNM staging and was higher in the patient group in clinical remission.
Conclusions:
The therapy of colon and rectum tumors based on microRNAs remains under investigation reserving huge potential for future applications and clinical interventions in conjunction with existing therapies. We expect, based on the exposed data, to stimulate the development of new therapeutic possibilities, making the treatment of these tumors more effective.
Keywords :
Colonic Neoplasms , Rectal Neoplasms , Genes , erbB-1 , MicroRNAs
