Author/Authors :
Zaragoza Bastida, Adrian Facultad de Medicina - Universidad Autonoma del Estado de Mexico - Paseo Tollocan/Jesus Carranza s/n, Mexico ´ 2 , Rivero Pérez, Nallely Area Academica de Medicina Veterinaria y Zootecnia - Instituto de Ciencias Agropecuaria, ´ Universidad Autonoma del Estado de Hidalgo - Av. Universidad Km 1 - Ex-Hda. de Aquetzalpa, Mexico , Valladares Carranza, Benjamín Centro de Investigacion y Estudios Avanzados en Salud Animal - Facultad de Medicina Veterinaria y Zootecnia - Universidad Autonoma del Estado de Mexico, Mexico , Isaac-Olivé, Keila Facultad de Medicina - Universidad Autonoma del Estado de Mexico - Paseo Tollocan/Jesus Carranza s/n, Mexico ´ 2 , Moreno Pérez, Pablo Facultad de Medicina - Universidad Autonoma del Estado de Mexico - Paseo Tollocan/Jesus Carranza s/n, Mexico ´ 2 , Sandoval Trujillo, Horacio Departamento de Sistemas Biologicos - Universidad Autonoma Metropolitana-Xochimilco, Mexico , Ramírez Durán, Ninfa Facultad de Medicina - Universidad Autonoma del Estado de Mexico - Paseo Tollocan/Jesus Carranza s/n, Mexico ´ 2
Abstract :
Mycobacterium genus causes a variety of zoonotic diseases. The best known example is the zoonotic tuberculosis due to M. bovis. Much less is known about “nontuberculous mycobacteria (NTM),” which are also associated with infections in humans. The Mexican standard NOM-ZOO-031-1995 regulates the presence of M. bovis in cattle; however, no regulation exists for the NTM species. The objective of this study was to isolate and identify nontuberculous mycobacteria species from cattle of local herds in the south region of the State of Mexico through the identification and detection of the 100 bp molecular marker in the 23S rRNA gene with subsequent sequencing of the 16S rRNA gene. Milk samples (35) and nasal exudate samples (68) were collected. From the 108 strains isolated, 39 were selected for identification. Thirteen strains isolated from nasal exudates amplified the 100 bp molecular marker and were identified as M. neoaurum (six strains), M. parafortuitum (four strains), M. moriokaense (two strains), and M. confluentis (one strain). Except M. parafortuitum, the other species identified are of public health and veterinary concern because they are pathogenic to humans, especially those with underlying medical conditions.
