Author/Authors :
Nunes, Vinicius Rodrigues Taranto Medical School - Universidade Federal de Minas Gerais (UFMG), Belo Horizonte-MG, Brazil , Vidigal, Paula Vieira Teixeira Medical School - Universidade Federal de Minas Gerais (UFMG), Belo Horizonte-MG, Brazil , Pereira, Márcio Tadeu Centro de Desenvolvimento da Tecnologia Nuclear (CDTN) - UFMG, Belo Horizonte-MG, Brazil , Ladeira, Luís Carlos Duarte Centro de Desenvolvimento da Tecnologia Nuclear (CDTN) - UFMG, Belo Horizonte-MG, Brazil , Barbuto, Rafael Calvão Medical School - UFMG, Belo Horizonte-MG, Brazil , Duval-Araujo, Ivana Medical School - UFMG, Belo Horizonte-MG, Brazil
Abstract :
Purpose:
To describe a new model of actinic enteritis that does not use radiotherapy machines.
Methods:
Sixteen Wistar rats were divided into four groups, consisting of four animals each: control (group A), two weeks after irradiation (group B), five weeks after irradiation (group C) and eight weeks after irradiation (group D). Animals were given a 10Gy radiation from a Cobalt-60 natural source in a nuclear technology research center. Protections of the surrounding tissues were obtained through the usage of plumb devices with a hole in the center, which served as a collimator. We obtained irradiated and non-irradiated colons from each animal.
Results:
In group B we found an important inflammatory response in the irradiated colon, which appeared in a reduced way in group C and was minimal in group D, in which we found a relevant collagen submucosal deposition/fibrosis. In all groups, the non-irradiated colon had a lower pathological damage in comparison with the irradiated colon.
Conclusion:
We thus described an efficient and feasible technique for obtaining an animal model of actinic enteritis.
Keywords :
Radiotherapy , Radiation , Enteritis , Inflammation , Fibrosis , Rats
