Title of article :
Biophysical Insight into the Interaction of Human Lysozyme with Anticancer Drug Anastrozole: A Multitechnique Approach
Author/Authors :
Almutairi, Fahad M Physical Biochemistry Research Laboratory - Biochemistry Department - Faculty of Science - University of Tabuk - P.O. Box 741 - Tabuk 71491 - Saudi Arabia , Rehan Ajmal, Mohammad Physical Biochemistry Research Laboratory - Biochemistry Department - Faculty of Science - University of Tabuk - P.O. Box 741 - Tabuk 71491 - Saudi Arabia , Al Alawy, Adel Ibrahim Physical Biochemistry Research Laboratory - Biochemistry Department - Faculty of Science - University of Tabuk - P.O. Box 741 - Tabuk 71491 - Saudi Arabia , Hasan Khan, Rizwan Interdisciplinary Biotechnology Unit - Aligarh Muslim University - Aligarh - India , Abdelhameed, Ali Saber Faculty of Pharmacy - King Saud University - Riyadh - Saudi Arabia
Abstract :
In the present study, we employ fluorescence spectroscopy, dynamic light scattering, and molecular docking methods. Binding of anticancer drug anastrozole with human lysozyme (HL) is studied. Binding of anastrozole to HL is moderate but spontaneous. There is anastrozole persuaded hydrodynamic change in HL, leading to molecular compaction. Binding of anastrozole to HL also decreased in vitro lytic activity of HL. Molecular docking results suggest the electrostatic interactions and van der Waals forces played key role in binding interaction of anastrozole near the catalytic site. Binding interaction of anastrozole to proteins other than major transport proteins in blood can significantly affect pharmacokinetics of this molecule. Hence, rationalizing drug dosage is important. This study also points to unrelated effects that small molecules bring in the body that are considerable and need thorough investigation.
Keywords :
human lysozyme (HL) , Biophysical Insight , Anticancer Drug Anastrozole , Multitechnique Approach
Journal title :
The Scientific World Journal
