Author/Authors :
Vagapova, E.R Engelhardt Institute of Molecular Biology - Russian Academy of Sciences, Moscow, Russia , Lebedev, T.D Engelhardt Institute of Molecular Biology - Russian Academy of Sciences, Moscow, Russia , Popenko, V.I Engelhardt Institute of Molecular Biology - Russian Academy of Sciences, Moscow, Russia , Leonova, O.G Engelhardt Institute of Molecular Biology - Russian Academy of Sciences, Moscow, Russia , pirin, P.V Engelhardt Institute of Molecular Biology - Russian Academy of Sciences, Moscow, Russia , Prassolov, V.S Engelhardt Institute of Molecular Biology - Russian Academy of Sciences, Moscow, Russia
Abstract :
The mechanism of resistance of leukemia cells to chemotherapeutic drugs remains poorly understood.
New model systems for studying the processes of malignant transformation of hematopoietic cells are needed.
Based on cytokine-dependent murine acute myeloid leukemia (AML) FDC-P1 cells, we generated a new cell
line with ectopic expression of the KIT gene encoding mutant human receptor tyrosine kinase (N822K). We
investigated the role played by overexpression of the mutant KIT in the survival of leukemia cells and their
sensitivity to therapeutic drugs. We also generated a co-culture system consisting of FDC-P1 murine leukemia
cells and a HS-5 human stromal cell line. Our data can be used for a further comprehensive analysis of the role of
KIT N822K mutation in the cellular response to anti-leukemic drugs, growth factors, and cytokines. These data
are of interest in the development of new effective therapeutic approaches to the treatment of acute leukemia.
Keywords :
stromal cells , KIT N822K , acute myeloid leukemia (AML) , FDC-P1 , receptor tyrosine kinase KIT
