Author/Authors :
Todorova, Biliana Vaccine Research Institute (VRI) - Creteil, France , Salabert, Nina Vaccine Research Institute (VRI) - Creteil, France , Tricot, Sabine Vaccine Research Institute (VRI) - Creteil, France , Boisgard, Raphaël Institute of Biomedical Imaging (I2BM) - Orsay, France , Rathaux, Mélanie Vaccine Research Institute (VRI) - Creteil, France , Grand, Roger Le Vaccine Research Institute (VRI) - Creteil, France , Chapon, Catherine Vaccine Research Institute (VRI) - Creteil, France
Abstract :
We developed a new approach to visualize skin Langerhans cells by in vivo fluorescence imaging in nonhuman primates.
Procedures. Macaques were intradermally injected with a monoclonal, fluorescently labeled antibody against HLA-DR molecule and
were imaged for up to 5 days by fibered confocal microscopy (FCFM). Results. The network of skin Langerhans cells was visualized
by in vivo fibered confocal fluorescence microscopy. Quantification of Langerhans cells revealed no changes to cell density with time.
Ex vivo experiments confirmed that injected fluorescent HLA-DR antibody specifically targeted Langerhans cells in the epidermis.
Conclusions. This study demonstrates the feasibility of single-cell, in vivo imaging as a noninvasive technique to track Langerhans
cells in nontransgenic animals.
