Author/Authors :
Fursova, N.A Institute of Gene Biology Russian Academy of Sciences, Moscow, Russia , Mazina, M.Y Institute of Gene Biology Russian Academy of Sciences, Moscow, Russia , Nikolenko, J.V Institute of Gene Biology Russian Academy of Sciences, Moscow, Russia , Vorobyova, N.E Institute of Gene Biology Russian Academy of Sciences, Moscow, Russia , Krasnov, A.N Institute of Gene Biology Russian Academy of Sciences, Moscow, Russia
Abstract :
In this work, we conducted a genome-wide study of the zinc finger protein CG9890 and showed that it is localized mostly on the promoters of active genes. The CG9890 binding sites are low-nucleosome-density regions and are colocalized with the chromatin modifying and remodeling complexes SAGA and dSWI/SNF, as well as with the ORC replication complex. The CG9890 protein was shown to be involved in the regulation of the expression of some genes on the promoters of which it is located, with the ecdysone cascade genes accounting for a significant percentage of these genes. Thus, the CG9890 protein is a new member of the transcriptional network which is localized on active promoters, interacts with the main transcription and replication complexes, and is involved in the regulation of both basal and inducible transcription.
Keywords :
ChIP-Seq , zinc fingers , Drosophila , CG9890 , ENY2
