Author/Authors :
Santos-Júnior, Luciano Department of Morphology and Structural Biology - Universidade Tiradentes (UNIT), Aracaju-SE, Brazil , Oliveira, Tassia Virgínia de Carvalho Department of Morphology and Structural Biology - Universidade Tiradentes (UNIT), Aracaju-SE, Brazil , Cândido, Janaína Farias Department of Morphology and Structural Biology - Universidade Tiradentes (UNIT), Aracaju-SE, Brazil , Santana, Danielle Souza de Department of Morphology and Structural Biology - Universidade Tiradentes (UNIT), Aracaju-SE, Brazil , Pereira, Rose Nely Department of Morphology and Structural Biology - Universidade Tiradentes (UNIT), Aracaju-SE, Brazil , Pereyra, Beatriz Benny Sungaila Department of Morphology and Structural Biology - Universidade Tiradentes (UNIT), Aracaju-SE, Brazil , Gomes, Margarete Zanardo UNIT, Aracaju-SE, Brazil , Lima, Sônia Oliveira UNIT, Aracaju-SE, Brazil , Albuquerque-Júnior, Ricardo Luiz Cavalcanti de UNIT, Aracaju-SE, Brazil , Cândido, Edna Aragão Farias UNIT, Aracaju-SE, Brazil
Abstract :
Purpose:
To investigate the cellular response to injury, analyzing histopathologic changes associated with increased cellularity, degeneration and disorganization of collagen fibers.
Methods:
Thirty wistar rats were divided in two groups after partial Achilles tenotomy: the right hind paw were treated with the essential oil of Alpinia zerumbet (EOAz), diluted to 33% (0.3 mL kg-1), and the left hind paw received sunflower oil for 3, 14, 30 and 90 days. Statistical significance was determined using a Chi-square and Pearson Correlation qualitative variables test. Moreover, Mann-Whitney U-test test for comparison between different groups of the same cell, one-way ANOVA, and Tukey’s test of quantitative measurement.
Results:
A decrease hyperemia (p < 0.001) was observed in the acute phase of inflammatory cell number (p < 0.001), whereas sub-acute phase was marked by significant correlation with macrophages in fibroblasts (r = 0.17, p = 0.03), with probable induction a dense and modeled tissue. At chronic phase, it was found an increase in the number of fibroblasts and a higher percentage of type I collagen fibers (78%) compared with control collagen fibers (55%).
Conclusion:
Oil of Alpinia zerumbet stimulated the process of maturation, organization and tissue repair which gave it greater resistance.
Keywords :
Achilles Tendon , Alpinia , Inflammation , Wound Healing , Phytotherapy , Rats
